zbio.net - main page Zbio.net > Protocols > Solutions > Single component Rambler's Top100  Russian version  Deutsche Version  Український варіант
MAIN PAGE · PROJECT · PROTOCOLS · PROGRAMS · LITERATURE · WEB · COMPANIES · MARKETPLACE · CLASSIFIED · FORUMS ·

Single component solutions

Densities of some solutions are available on the page "Densities of acids, alkali and organic substances".





    About the recalculation of recipes for the arbitrary volumes:


    Organic substances

    pKa and temperature dependence of pH for common buffers

    BufferMwpKa
    20oC
    Working
    range
    delta pKa
    per 10oC
    MES
    2-(N-morpholino)ethanesulfonic acid
    195.26.155.5-6.7-0.110
    Bis-Tris
    bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane
    209.26.55.8-7.2 
    ADA
    N-(2-acetamido)-2-imidoacetic acid
    190.26.606.0-7.2-0.110
    PIPES
    piperasine-N,N'-bis(2-ethanesulfonic acid)
    302.46.806.1-7.5-0.085
    ACES
    2-[(2-amino-2-oxoethyl)amino]ethanesulfonic acid
    182.26.906.1-7.5-0.200
    MOPSO
    3-(N-morpholino)-2-hydroxypropanesulfonic acid
    225.36.96.2-7.6 
    Imidazol - HCl68.086.956.2-7.8 
    Bis-Tris Propane
    1,3-bis[tris(hydroxymethyl)methylamino]propane
    282.36.8*6.3-9.5 
    BES
    N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid
    213.27.156.4-7.8-0.160
    MOPS
    3-(N-morpholino)propanesulfonic acid
    209.37.206.5-7.9-0.013
    TES
    N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid
    229.27.506.8-8.2-0.200
    HEPES
    N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
    238.37.556.8-8.2-0.140
    DIPSO
    3-[N,N-bis(2-hydroxyethyl)amino]-2-hydroxyprpanesulfonic acid
    243.37.67.0-8.2 
    TAPSO
    3-[N-tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic acid
    259.37.67.0-8.2 
    HEPPSO
    N-(2-hydroxyethyl)piperazine-N'-(2-hydroxypropanesulfonic acid)
    268.37.87.1-8.5 
    POPSO
    piperazine-N,N'-bis(2-hydroxypropanesulfonic acid)
    362.47.87.2-8.5 
    TEA
    triethanolamine
    149.27.87.3-8.3 
    EPPS
    N-(2-hydroxyethyl)piperazine-N'-(3-propanesulfonic acid)
    252.38.07.3-8.7 
    Tricine
    N-tris(hydroxymethyl)methylglycine
    179.28.157.4-8.8-0.210
    Tris (TRIZMA)
    tris(hydroxymethyl)aminomethane
    121.18.307.0-9.1-0.310
    Bicine
    N,N-bis(2-hydroxyethyl)glycine
    163.28.357.6-9.0-0.180
    TAPS
    N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid
    243.38.47.7-9.1 
    Glycylglycine 8.40 -0.280
    AMPSO
    3-[(1,1-dimethyl-2-hydroxyethyl)amino]-2-hydroxypropanesulfonic acid
    227.39.08.3-9.7 
    CHES
    1-(N-cyclohexylamino)ethanesulfonic acid
    207.39.38.6-10.0 
    CAPSO
    3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid
    237.39.68.9-10.3 
    AMP
    2-amino-2-methyl-1-propanol
    89.19.79.0-10.5 
    CAPS
    3-(cyclohexylamino)-1-propanesulfonic acid
    221.310.49.7-11.1 

    * pKa=9.0 for the second stage of dissociation.

    ATP

    C10H14N5O13P3Na2; Mw=551.1 g/M
    store at -20o
    Conc.Stock1ml
    ATP0.1M551.1g/M55.1mg
    H2O mQ  

    Unchecked information 0.1M ATP (pH 7.2): 5.71% (w/w) ATP, 84.86% (w/w) H2O, 9.43% (w/w) 2M Tris base.

    1. adjust to pH7.5 by 2M NaOH (~70-80µl);
    2. prepare about 700µl, dilute 2000 times (it is the final dilution in the spectrofotometric cell), check the optical density:
    3. C[M]=A259/15.4x103=A259 1:2000x0.130

    4. adjust the final volume.

    Betaine

    monohydrate C5H11NO2xH2O; Mw=135.2g/M;
    store at 4oC
    Conc.Stock%(w/w)100ml200ml700ml
    Betaine135.2g/M5M63.0067.6g135.2g473.2g
    H2O mQ37.0039.74ml79.49ml278.2ml

    p~1.073g/ml


    Cresol red (Na)

    store at +4, -20oC
    Conc.Stock1ml50ml
    Cresol red (Na)50mM404.4g/M20.2mg1.01g
    H2O mQ   
  • Cresol Red (Na salt) is a very convenient dye. At a concentration of about 0.2mM it is compatible with restriction digestion, PCR, sequencing. It may be used as marker for electrophoresis;
  • the color of the dye is pH-dependent (red, if pH>7.5; yellow, if pH <7.0). It is possible to use Cresol Red as pH-indicator (i) for denaturation of double-stranded DNA in sequencing, (ii) for silica-purification of DNA from agarose gel.

  • DTT

    C4H10O2S2; Mw=154.2g/M
    store at -20oC

    Conc.Stock%(w/w)1ml5ml10ml20ml
    DTT154.2g/M1M14.550.155g0.773g1.55g3.09g
    AcNa, pH 5.2 10mM85.45905 µl4.53ml9.05ml18ml

    p~1.06g/ml

    Conc.Stock1ml5ml10ml20ml
    DTT154.2g/M2.2M0.339g1.696g3.394g6.787g
    AcNa, pH 5.2 10mM     
  • filter sterililize;
  • no DEPC treatment.


See also:
/web-links/

    Preparation of dNTP's.

    The quick protocol for ~100mM stock

    1. dilute all four dNTP's (250mg of each) in 3.676ml of H2O;
    2. + 424µl 5M NaOH;
    3. check the pH: ~0.5µl on pH-paper.

    Accurate protocol for 100mM stocks preparation

    1. add the necessary quantity (see table) of H2O and Tris base 1M (it is possible to take the volume of salt as ~150µl);
    2.    

      Mw

      V(H2O)V(Tris base 1M)V(final)
      dATPC10H14N5O12P3Na2x3H2O589.23.24ml850 µl4.24ml
      dGTPC10H13N5O13P3Na3x2H2O609.23.55ml400 µl4.10ml
      dCTPC9H13N3O13P3Na3x2H2O569.13.44ml800 µl4.39ml
      dTTPC10H14N2O14P3Na3x2H2O584.13.73ml400 µl4.28ml
    3. check the pH: ~0.5µl on pH-paper;
    4. check the quality and concentration (it is useful to take final dilution 1:5000 (~20µM). In this case the optical density will be in the region of Am~0.3 - the most accurate range for spectrophotometer).
      Concentration is c[mM]=k1:5000xAm.

    Quality:

    dATP
    pH 7.0
    A250/A260=0.80+0.03
    A280/A260=0.12+0.02
    dCTP
    pH 2.0(!)
    A250/A260=0.45+0.03
    A280/A260=2.10+0.15
    A290/A260=1.60+0.10
    dGTP
    pH 7.5
    A250/A260=1.18+0.04
    A280/A260=0.67+0.03
    A290/A260=0.28+0.03
    dTTP
    pH 7.5
    A250/A260=0.65+0.03
    A280/A260=0.73+0.03

    Concentration:

      MwAmpHEk (for 1:5000)
    dATP[Na2]589.22597.015.2x103328.9
    dGTP[Na3]609.22537.513.7x103365.0
    dCTP[Na3]569.12802.0 (!!!)13.0x103384.6
    dTTP[Na3]584.12677.59.6x103520.8
           
    ATP [Na4]595.1259 15.4x103 
    CTP [Na4]571.1280 13.0x103 
    GTP [Na4]611.1252 13.7x103 
    UTP [Na4]572.1262 10.2x103 

    Concentration: c[M]=Amax/E; Amax = maximum of absorption.



    EDTA

    C10H14O8N2Na2x2H2O; Mw=372.3g/M; pH = 8.0
    store at 4oC
    Conc.Stock%(w/w)50 ml100 ml150 ml250 ml
    EDTA0.5 M372.3g/M16.989.31g18.62g27.92g46.55g
    NaOH~0.5 M40g/M1.821.014g2.028g3.042g5.07g
    H2O mQ81.1944.48ml88.95ml133.4ml222.4ml
    Conc.Stock10 ml50 ml100 ml150 ml250 ml
    EDTA0.5 M372.3g/M1.86g9.31g18.62g27.92g46.55g
    NaOH~0.5 M10M 507µl
    0.674g
    2.535ml
    3.37g
    5.07ml
    6.74g
    7.61ml
    10.11g
    12.68ml
    16.85g

    H2O mQ8.42ml42.12ml84.24ml126.4ml210.6ml

    p~1.096g/ml

  • EDTA is not soluble at acidic pH; it is necessary to add alkali gradually and to control pH;
  • do not treat by DEPC.

  • EtBr

    Ethidium bromide, C21H20N3Br; Mw=394.3g/M
    store at NT in the dark
    Conc.5ml10ml50ml
    EtBr10mg/ml50mg100mg500mg
  • soluble in H2O, EtOH, chloroform;
  • concerning carcinogenic properties of EtBr. The only data that we found in the literature is that in mutagenic test (on bacteria) 90µg of EtBr gave the same results as the smoke concentrate from one cigarette.

  • Gelatin

    store at 4oC

    Conc.Stock10ml50ml100ml
    Gelatin2%solid0.2g1.0g2.0g
    H2O mQ    
  • sterilize by autoclaving.

  • Glucose

    C6H12O6xH2O; Mw=198.17g/M
    store at 4oC
    Conc.Stock%(w/w)50ml100ml250ml
    Glucose2M198.17g/M34.97219.82g39.63g99.09g
    H2O mQ65.02836.85ml73.70ml184.24ml
    1000ml/
    198.17g/M1M1.5M2M
    Glucose [g]198.17
    297.255
    396.34
    H2O [ml]868.23
    802.745
    736.96
    %(w/w)18.58327.02334.972
    H2O %(w/w)81.41772.97765.028
    p (g/ml) 20oC1.06641.11.1333


    Guanidine HCl (GuHCl)

    CH5N3xHCl; Mw=95.53g/M

    Molarity; 1000ml /
    12345678
    GuHCl95.53g
    191.06g
    286.59g
    382.12g
    477.65g
    573.18g
    668.71g
    764.24g
    H2O (mQ)924.2ml
    854ml
    783.7ml
    711.7ml
    639.8ml
    567.2ml
    494.3ml
    420.7ml
    GuHCl %(w/w)9.3718.2826.7834.9342.7550.2657.5064.50
    H2O %(w/w)90.6381.7273.2265.0757.2549.7442.5035.50
    p (g/ml)1.0201.0451.0701.0941.1171.1401.1631.185
  • solubility: at 25oC - 8.54M, 5oC - >8M;
  • A260(6M in H2O)<0.03;
  • it is possible to take the "partial density of GuHCl" as 0.763 in calculations of solutions.


  • HEPES

    Conc.Stock%(w/w)1L

    C8H18N2O4S

    1M238.3g/M22.40238.3g
    H2O mQ77.60825.7ml

    p=1.064

    HEPES, 1M, 1L

    Desired pHKOH, 5M
    [1000ml]
    5.250ml0ml
    5.350.5ml
    5.753.5ml
    6.037ml
    6.2412ml
    6.5922ml
    6.7132ml
    6.8845ml
    7.0050ml
    7.1060ml
    7.2580ml
    7.3792.5ml

    Imidazol

    C3H4N2
    store at 4oC

    Conc.Stock50ml100ml
    Imidazole2M68.1g/M6.81g13.62g
    H2O mQ   

    Paraformaldehyde

    PFA (paraformaldehyde) 37% (for histochemistry it should be freshly prepared).

    1. mix in the screw-cap tube:

    PFA (solid) = 0.37g,
    H2O = 1.0ml
    NaOH (1N) = 14µl;

    2. solubilize in the boiling water bath (to heat ~1-3'; until pH will drop to ~7.0).


    PEG

    H(OCH2CH2)nOH
    store at 4oC

    Conc.%(w/w)10ml50ml100ml150ml200ml
    PEG600040%37.214.0g20g40g60g80g
    H2OmQ62.796.75g33.75g67.5g101.25g135.0g

    p=1.075.


    PMSF

    store at -20oC

    Conc.Stock20ml
    C7H7FO2S100mM174.2g/M0.348g
    Isopropanol  20ml

    Retinoic acid

    all trans-Retinoic acid, Tretinoin, light-sensitive
    store at -20oC

    Conc.Stock16.6ml
    C20H28O210mM300.4g/M50mg
    EtOH >96%16.6ml
  • stock solution is 10mM, working solution is freshly prepared 1mM in EtOH (it would be better to add pure EtOH to the control cells).

  • Sucrose

    C12H22O11, Mw=342.30g/M
    store at 20oC

    Densities and refraction indexes of sucrose solutions.
    Conc.Stock%(w/w)50ml100ml250ml
    Sucrose1M342.30g/M30.33017.115g34.23g85.58g
    H2O mQ69.67039.315ml78.63ml196.58ml
    1000ml/
    342.30g/M1M2M2.5M
    Sucrose [g]342.3
    684.6
    855.75
    H2O [ml]786.3
    570.4
    460.35
    %(w/w)30.33054.55065.022
    H2O %(w/w)69.67045.45034.978
    p (g/ml) 18oC1.12861.25501.3161

    Tris Cl

    C4H11O3N; Mw=121.1g/M
    store at 4oC
    Conc.Stock50 ml100 ml150ml200ml
    Tris-base1M121.1g/M6.0612.11g18.17g24.22g
    H2O to the final weight mQ52.03g104.06g156.09g208.12g

    1M: p=1.0406

    Unchecked information 2M Tris base: 22.90%(w/w) Tris base, 77.10%(w/w) H2O; p=1.058

  • do not treat by DEPC;
  • sterilize by autoclaving;
  • pH of Tris-buffers is dependent from concentration. If to take 50mM solution as the original:
  • pH(500mM) => + 0.05
    pH(5mM) => - 0.05

  • pH drops on 0.028 when the temperature rise on 1oC.
  • Temperature dependence of pH for Tris Cl 50mM.

    pH at g/50ml 1M or
    g/liter for 0.05 M
       
    5oC25oC37oC Tris HClTris BaseH2O Tris HClTris BaseH2O
    7.557.006.70 7.280.4744.28
    7.667.106.80 7.130.5744.33
    7.767.206.91 7.020.6744.34
    7.897.307.02 6.850.8044.38
    7.977.407.12 6.610.9744.45
    8.077.507.22 6.351.1844.50
    8.187.607.30 6.061.3944.58
    8.267.707.40 5.721.6644.65
    8.377.807.52 5.321.9744.74
    8.487.907.62 4.882.3044.85
    8.588.007.71 4.442.6544.94
    8.688.107.80 4.022.9745.04
    8.788.207.91 3.543.3445.15
    8.888.308.01 3.073.7045.26
    8.988.408.10 2.644.0345.36
    9.098.508.22 2.214.3645.46
    9.188.608.31 1.834.6545.55
    9.288.708.42 1.504.9045.63
    9.368.808.51 1.235.1345.67
    9.478.908.62 0.965.3245.75
    9.569.008.70 0.765.4745.80
    9.679.108.79 0.695.5345.81

    TrisCl: 250ml 1M

    V HClV HClpH
     010.44
     19.54
     29.26
     39.08
     3.78.98
    5ml 8.76
    6ml 8.67
     108.4
    11ml 8.22
     148.13
    13.5ml 8.06
    14ml 8.04
    15ml 7.97
     177.92
    16.5ml 7.82
    16.7ml 7.8
    17ml 7.77
    18.3ml 7.63
    18.6ml 7.59
     207.75
     237.4


    Tricine

    C6H13NO5; Mw=179.2g/M
    store at 4oC
    Conc.Stock50ml
    Tricine1M179.2g/M8.96g
    H2O mQ  

    For 50ml:   

    Desired pH5N KOH
    [50ml]
    8.307.0ml
    8.387.5ml
    8.478.0ml
    8.58.15ml
    8.68.5ml
    8.689.0ml
    8.789.5ml
    8.9010.0ml

    Triethanolamine

    1M
    store at 4oC
    Conc.Stock%(w/w)50ml
    Triethanolamine1M149.19g/M14.697.46g
    6.66ml

    H2O mQ85.3143.34ml

    Urea

    CH4N2O; Mw=60.06g/M
    Molarity; 1000ml /
    12345678910

    CH4N2O
    [g]

    60.06
    120.12
    180.18
    240.24
    300.30
    360.36
    420.42
    480.48
    540.54
    600.60
    H2O
    [ml]
    950.6
    905.8
    861.3
    817.0
    771.6
    726.7
    681.2
    635.7
    590.0
    544.1
    p (g/ml)1.0111.0261.0411.0571.0721.0871.1021.1161.1311.145

    CH4N2O
    %(w/w)

    5.9411.7117.3022.7228.0233.1538.1643.0547.8152.47
    H2O %(w/w)94.0688.2982.7077.2871.9866.8561.8459.9552.1947.53
  • solubility: at 25oC: 10.49M, 5oC: ~8M;
  • A260(6M in H2O)<0.06;
  • it is possible to take the "partial density of urea" as 0.763 in calculation of solutions.


  • Acids and alkalis

    Name:Formula:Mw% (w/w)[M]g in 1L
    of subst.
    p [g/ml]ml/L for
    1M sol.
    Sodium hydroxideNaOH4050%19.17631.5352.4
    30.1%10.04001.329100
    10%2.751111.11363.6

    Potassium hydroxide

    KOH

    56.150%13.57571.5274.1
    23.06%5.0280.61.217200
    10%1.941091.09515.5
    Ammonium hydroxideNH4OH35.028%14.82510.89867.6
    Acetic acid, glacialCH3COOH60.0599.5%17.410451.0557.5
    Acetic acid36%6.273761.045159.5
    Formic acidHCOOH46.0290%23.410801.2042.7
    Hydrochloric acidHCl36.536%11.64241.1886.2
    10%2.91051.05344.8
    Nitric acidHNO363.0271%15.9910081.4262.5
    67%14.99381.4067.1
    61%13.38371.3775.2
    Perchloric acidHClO4100.570%11.6511721.6785.8
    60%9.29231.54108.7
    Phosphoric acidH3PO498.085%18.114451.7155.2
    Sulfuric acidH2SO498.196%18.017661.8455.6

    NaOH

    Mw=40g/M
    store at NT
    Conc.Stock%(w/w)50ml200ml300ml
    NaOH10M40g/M30.120g80g120g
    H2O mQ69.9046.45ml185.8ml278.7ml

    10M: 30.10%; p=1.329.

    Conc.Stock%(w/w)50ml150ml200ml
    NaOH1M10M12.875ml
    6.645g
    15ml
    19.94g
    20ml
    26.58g

    H2O mQ87.1345ml135ml180ml
  • it is better to use plastic bottles for storage, because the alkali slightly solubilize the glass.

  • KOH

    Mw=56.11g/M
    store at NT

    Conc.Stock%(w/w)10ml50ml100ml150ml
    KOH5M56.11g/M23.052.806g14.03g28.06g42.08g
    H2O mQ76.959.363g46.81g93.63g140.4g

    5M: 23.06%; p=1.217.

  • it is better to use plastic bottles for storage, because the alkali slightly solubilize the glass.

  • TCA

    CCl3CO2H; Mw=163.39g/M
    store at NT, in the dark, under the fume hood
    Conc.%(w/w)5ml10ml25ml50ml
    TCA100% (w/v)68.785.0g10.0g25.0g50.0g
    H2OmQ31.222.27ml4.54ml11.35ml22.7ml


    Detergents

    DetergentTmeltMw [Da]CMC
    monomermicelle%(w/v)M
    Anionic
    SDS20628818,0000.238.0 x 10-3
    Cholate2014304,3000.601.4 x 10-2
    Deoxycholate1754324,2000.215.0 x 10-3
    Cationic
    C16TAB23036562,000.041.0 x 10-3
    Amphoteric
    LysoPC-49592,0000.00047.0 x 10-6
    CHAPS1576156,1500.491.4 x 10-3
    Zwittergent 3-14-36530,0000.0113.0 x 10-4
    Nonionic
    Octyl glucoside1052928,0000.732.3 x 10-2
    Digitonin2351,22970,000--
    C12E8-54265,0000.0058.7 x 10-5
    Lubrol PX-58264,0000.0061.0 x 10-4
    Triton X-100-65090,0000.0213.0 x 10-4
    Nonident P-40-60390,0000.0173.0 x 10-4
    Tween 80-1,31076,0000.0021.2 x 10-5


    CMC - critical micelle concentration.

    Detergent123456789101112 1 - Strongly denaturing;
    2 - Dializable;
    3 - Ion exchangeable, unsuitable for ion-exchange chromatography;
    4 - Complexes ions;
    5 - Strong A280;
    6 - Assay interference;
    7 - Cold precipitates;
    8 - High cost;
    9 - Ease of purification;
    10 - Radiolabeled;
    11 - Definite composition;
    12 - Auto-oxidation
    Anionic
    SDS++++--+-+++-
    Cholate-+++----+++-
    Deoxycholate-+++--+-+++-
    Cationic
    C16TAB+++---+-+-+-
    Amphoteric
    LysoPC+/-------++/-++-
    CHAPS-+-----++-+-
    Zwittergent 3-14+/-+/------++-+-
    Nonionic
    Octyl glucoside-+-----+-++-
    Digitonin-------++-  
    C12E8---+/----+-+-+
    Lubrol PX---+/--+/----+-+
    Triton X-100---+/-++/----+-+
    Nonident P-40---+/-++/----+-+
    Tween 80---+/--+/----+-+
    C16TAB - hexadecyl trimethylammonium bromide;
    LysoPC - lysophosphatidylcholine.
    * Sodium cholate and sodium deoxycholate are unsoluble at <pH 7.5 or at ionic strength greater then 0.1%. SDS may precipitate below 20oC;
    ** Ionic detergents may induce problems with electrophoresis and isoelectric focusing;
    *** It is possible to remove by dialysis;
    **** Phenol -containing detergents (for example Triton X-100 and NP-40) precipitate during Folin protein assay (but do not interfere with Bradford protein assay).


    N-Lauroylsarcosine Na

    store at NT
    Conc.Stock%(w/w)1ml3ml5ml15ml50ml
    Sarc10%30%33.930.333ml
    0.342g
    1.00ml
    1.027g
    1.67ml
    1.712g
    5.00ml
    5.14g
    16.67ml
    17.12g

    H2O mQ66.070.667ml2.00ml3.33ml10.00ml33.33ml

    p=1.017.


    SDS

    Sodium dodecyl sulfate, sodium lauryl sulfate; [CH3(CH2)10CH2SO4]Na; Mw=288.4g/M
    store at NT
    Conc.Stock%(w/w)100 ml250 ml300 ml400 ml
    SDS10%(w/v)solid9.8210g25g30g40g
    H2O mQ90.1891.8 ml229.5 ml275.4 ml367.2 ml

    p = 1.018

  • weight under the fume hood (and better to wear the mask);
  • it is necessary to heat to 60-80oC. to facilitate solubilization. Check pH. If it differs from neutral (~7.2-7.5) dramatically - adjust by diluted alkali / acid.


  • Organic solvents

    Phenol

    C6H5OH; Mw=94.1g/M
    p=1.054, tm= 43, tb= 182, pKa=10.0
    solubility: 6.816H2O, unlimited 66H2O; unlimited EtOH

    Preparation of "acidic" and "neutral" phenol.

    a) from the distillation:

    1. distill phenol under H2O;
    2. adjust water to the volume of about 1/10 of phenol phase;
    3. add 8-hydroxyquinoline to 0.1% (relative to phenol phase) and bMeEtOH (2-mercaptoethanol) to 0.2% (relative to H2O = 0.02% relative to phenol phase);
    4. ---- on this step you obtained the "acidic phenol". Store at -20oC (~1 year); ----

    5. add about the same volume of 0.2 M Tris-base to the phenol, mix ~0.5-1h;
    6. throw away aqueous phase;
    7. + 0.1V 0.1M Tris-Cl, pH 8.0
    8. 0.2% bMeEtOH
    9. mix ~0.5-1h;
    10. store at 4oC (in the dark) ~several months.

    b) from the good commercial substance:

    1. saturate phenol by the water (add about 1/5 V );
    2. then, according to (a), from the p.2.

    ______________
    * For RNA extraction it is better to saturate (just add) "acidic phenol" by the following buffer: (store at 4oC):

    Conc.Stock100ml
    AcONa, pH 5.150mM3M1.67ml
    EDTA10mM0.5M2.0ml
    H2O mQ96.0ml
  • check pH and adjust by diluted (1:100) acetic acid if necessary.
  • Unchecked information there was note in the "Mitchell Group Methods" that acidic phenol is much more stable, then the neutral. They recommend preparing neutral phenol in small quantities only for a few weeks.


    EtOH

    CH3CH2OH; Mw=46.1g/M

    densities of aquatic solutions of ethanol.
  • very hygroscopic, (store at NT in tightly closed bottle);
  • tпл.=-117.3oC; tкип.=78.5oC.
    Conc.Stock300ml
    EtOH70%96%190g
    H2O mQ70ml

    Preparation of 100% EtOH.

    1. dry CuSO4 (at 65oC, 1-2 days) to the practically white color;
    2. mix about 1/4 of volume of the CuSO4 with 3/4 volume of 96%EtOH;
    3. mix intensively;
    4. leave for 1-2 days (CuSO4 will "take" water from ethanol).

    Unchecked information test for the "100%" ethanol: add a drop of ethanol to the xylene. No mist should appear in the case of 100% ethanol.



Zbio.net: http://zbio.net
e-mail: editor@zbio.net
seen: 36781

    Supplement





       
Last modification: 30/07/13

MAIN PAGE · PROJECT · PROTOCOLS · PROGRAMS · LITERATURE · WEB · COMPANIES · MARKETPLACE · CLASSIFIED · FORUMS ·

 ·  Викимарт - все интернет-магазины в одном месте  ·  Доска объявлений Board.com.ua  · 
--- сервер арендован в компании Hetzner Online, Германия ---
--- администрирование сервера: Intervipnet ---

zbio.net  ·  editor@zbio.net  ·  advertizing

molbiol.ru - методы, информация и программы для молекулярных биологов   Rambler