Liquids densities


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Densities of liquids

Comments

Supplement

Weight measurements are always much more precise than volume measurements. An electronic balance makes it not only more precise, but also more convenient.

Substance	Density [g/ml]	Additional information
Acetic acid glacial	1.049	T_b=118^oC
Acetic acid 36%	1.049
Acetone	0.788	T_b=56.5^oC
AcOK, 5.0M 1.0M	1.218 1.046
AcONa, 3.0M 2.5M	1.121 1.147
AcONH₄, 10M	1.073
Acrylamid:BIS, 40%	1.044
Ammonium hydroxide, NH₄OH, 28%	0.89
Blocking reag., 10%	1.038
Butanol (n)	0.81	T_b=117-118^oC
CaCl₂, 1M	1.086
Chloroform	1.48	T_b=61-62^oC
Denhardt sol., 50X	1.004
DMFA	0.945
DMSO	1.100
EDTA, 0.5M	1.096
Ethanol, anhydr 96% 70%	0.79 0.80 0.868	T_b=78.5^oC
FGRB, 50X	1.104
Formaldehyde, 37%	1.09
Formamid	1.133
Glucose, 10%(w/v) 2M	1.040 1.13
Glycerol	1.25
GTGB, 20X	1.094
HCl, 37% (12.07M)	1.19
Isoamyl alcohol	0.813	T_b=132^oC
Isobutanol	0.871	T_b=108^oC
Isopropanol	0.785	T_b=82.5^oC
KCl, 0.25M	1.044
KOH, 5.0M	1.217
LB	1.024
LiCl, 9M 8M	1.194 1.172
Mercaptoethanol (ß)	1.114	14.192M, Mw 78.13
MgCl₂, 1M	1.073
MgSO₄, 1M	1.112
MnCl₂, 1M	1.099
Nitric acid, HNO₃71%	1.42
NaCl, 5M	1.186
NaOH, 50%	1.53
NaOH, 10M	1.329
NaxHPO₄, 1M,pH~7	1.104
Nonident P-40	1.064
Octane	0.71	T_b=125.6^oC
PEG 6000, 20% 40%	1.041 1.075
PEG/NaCl, (20%/2.5M)	1.145
PEG/TAE, (15%/1X)	1.032
Phosphoric acid H₃PO₄, 85%	1.70
Phenol	1.054	T_b=182^oC, T_m=43^oC
Sulfuric acid H₂SO₄, 96%	1.84
Sarcosyl, 10%(w/v) 30%(w/v)	1.017 1.027
SDS, 10%	1.018
SSC, 20X 30X	1.163 1.245
SSPE, 20X	1.136
TAE, 50X	1.088
TBE, 10X	1.070
Tris.Cl, 1M, pH 7-8 2M, pH7.9	1.041 1.10
TritonX-100, 10%	1.016
TritonX-100, 100%	1.06
Tween 20, 100%	1.108 (1.101)
ZnCl₂, 1M	1.111

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Comments

We prefer to use a balance for preparing:

* agarose gels (weighing of agarose and buffer);
* AA gels (weighing of all components);
* 1x electrophoresis buffers from the stocks (weighing of water and stock solution);
* salt solutions;
* in the cases of viscous liquids, large volumes etc.

It is really convenient if there are two balances in the laboratory. One (that is used more often) is with the 0.01g gauge and the range to 1.5-3kg. It is used for preparation of large volumes of buffers, balancing of centrifuge tubes etc. The other balance has 0.0001g gauge and the range of 50-100g. It is used for preparation of small volumes of buffers (it is sufficient if the neighboring laboratory has such a scale).
If the density of the solution is known, it is possible to calculate the water volume (weight) that is necessary for its preparation. It is very convenient, because otherwise phrase "add water to the final volume…" forces you to use the graduated cylinder for the preparation of solution (calibration of all other laboratory glassware is only approximate).

See also:
/web-links/

Supplement

People who know! Some information, that you know, but the author did know (or forgot) is missed here. Your colleagues are using this page. Help them, please. Any information, which is helpful for the work are valuable.

Guest /28.12.2005 22:55/

what liquids do not readily dissolve in water subject to a contastant agitiation and are lighter so remain on the surface? (other than oil) preferable also that do not leave a film like oil does on thinngs

Guest /19.01.2006 21:59/

can i find the density of liuquids by making and using a straww hydrometer

n /01.02.2006 04:25/

Density and solubility are two different things.

Guest /06.02.2006 07:00/

ketchup

Guest /24.03.2006 05:56/

You should put densities of liquids peole might have heard of or have in their home. For example, honey, Mountain dew, Liquid soap, coffee, or Shampoo.

Guest /10.06.2008 17:52/

3MAcetic acid glacial

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