Guest /02.03.2009 01:25/

Really want to thank you for the hand drawings. For some reason, and even though Illumina has great pictures to explain, these hand drawings really break things down.

Guest /20.10.2009 09:30/

Excellent hand drawings, I struggled a lot to understand bridge PCR, because certain steps were never shown in diagrams. Shall be highly obliged if emulsion pcr is also illustrated the same way.

Guest /04.11.2009 15:33/

THANK YOU SO MUCH! maybe ill make my test today thanks yo you:D

Guest /28.11.2009 07:34/

Thanks for the drawings. But is it really necessary to cut one of the primers to remove all the strands of one orientation? It seems to me that if all the strands remain, then all the strands will give the same sequence with a particular primer, it's just that half will be sequenced from the substrate-proximal side, while the other half will be sequenced from the substrate-distal side.

I will probably use your drawings to show my class; I will credit them to "Soldatov" at your URL.

Guest /11.12.2009 08:30/

Could understand better on Illumina seq. Thanks a lot. Please keep updating this site with more info on all seq applications from Illumina, solid and 454

Guest /04.01.2010 12:16/

Guest /28.11.2009 07:34/ -
The technology only reads 50-100bp from the start of the strand (Illumina) while the total length of the strands is much longer - you are essentially envisioning paired end sequencing which utilize 2 primers to differentiate which side it is reading:
http://www.illumina.com/technology/paired_...cing_assay.ilmn ( http://www.illumina.com/technology/paired_end_sequencing_assay.ilmn )

Guest1 /06.01.2010 04:11/

the topic is called - "Second-Generation" Zumbajspil