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* Biochemical Spectroscopy -- Sauer K. --
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 Redactoradmin. Берлин, Германия  |
  13.06.2005 03:08
 Methods in Enzymology кн.246 Ред. Sauer K. Elsevier Science; 1995; 816стр.; ISBN: 0121821471 The use of spectroscopic methods to examine biomolecules has a long and rich history. Such methods have the virtue of being largely noninvasive and capable of probing living materials as well as subcellular preparations and isolated biomolecules. The information gained is interpretable in terms of structural parameters and intramolecular interactions. Using time-resolved approaches, dynamics can be explored readily over a time range from less than a picosecond to seconds and longer. This permits ready exploration of intermolecular interactions and intramolecular motion relevant to biological processes. Advances in technology and methodology in spectroscopy have moved the field forward at a breathtaking pace in recent years. We have come a long way from the era when cytochrome oxidation state changes were monitored visually using a hand spectroscope or when absorption spectrometry was done using photographic detection. In this volume the reader can learn about instrumentation that uses diode array detectors to monitor absorption or emission spectral properties with great precision at hundreds of wavelengths simultaneously or Fourier transform methods that provide a significant increase in the efficiency of collecting and analyzing spectroscopic information distributed over a wide wavelength band. Mode-locked lasers and associated pulse-compression and continuum- generation techniques allow pulse-probe measurements of fast (to l0 fsec = l0 -~4 sec) absorption changes or fluorescence relaxation in the picosecond regime. Single-photon counting methods have greatly improved the signal-to-noise of optical detection systems for steady-state spectroscopy and especially for time-resolved fluorescence measurements. Pulsed lasers have advanced the application of time-resolved Raman spectroscopy and the ability to discriminate between Raman and fluorescence signals. In combination with the use of ultralow temperatures, intense monochromatic laser sources can be used in hole-burning experiments to probe chromophore local environments and the modes by which the chromophores interact with their surroundings. Computation intensive methods, such as Fourier transform infrared, electron spin echo (ESE), pulsed electron nuclear doable resonance (ENDOR), and digital imaging optical spectroscopy, have provided entirely new approaches to data collection and processing. Major developments in radiation sources, such as synchrotrons, have opened entirely new areas of investigation, including X-ray absorption spectroscopy (XAS) and extended X-ray absorption fine structure (EXAFS). The reader will find descriptions and examples of each of these new methodologies in the chapters that follow. The audience for this volume includes the current generation of graduate students and professional scientists involved in biological or biochemical studies seeking an introduction to modern spectroscopic methods and instrumentation. To help the interested reader develop a deeper background and understanding of these methodologies and approaches, the authors of the individual chapters have cited general references and published reviews of the individual topics. The chapters fall into major sections covering optical spectroscopy, vibrational spectroscopy, electron paramagnetic resonance, and X-ray spectroscopy. Areas such as nuclear magnetic resonance which have been described extensively in recent volumes of this series have not been included. A general overview of the contents of this volume and examples of problems or situations to which the different approaches have been applied are described in the first chapter. |
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Приведённые здесь книги бродит по рукам в электронном виде. На тему о том, "где их взять": 1) прямые ссылки могут быть в комментариях к описанию книги; 2) можно поискать в известных онлайн библиотеках; 3) если первые два способа не помогают, можно 'попросить у коллег' (требуется регистрация!) NB! запросы на книги, которых в каталоге нет, можно делать только в теме "Книги которых нет, но хочется"; из всех других мест запросы удаляются без предупреждений! | ||
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afanasev-max Постоянный участник Irkutsk-Moscow-Irkutsk |
11.08.2005 14:57
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