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SynPony |
DNeasy – like Plant DNA Extraction Protocol (Buffer AP1) 10 mM Tris-HCl pH8.0 1 mM EDTA pH 8.0 0.1% SDS 0.1M NaCl +1X PVP +10mM DTT Скажи пожалуйста, что в данном случае означает 1X PVP Спасибо! |
dk Постоянный участник Россия |
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metrim Постоянный участник Москва |
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dk Постоянный участник Россия |
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-Ъ- Постоянный участник Москва |
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beadbeater24 |
(-Ъ- @ 26.04.2021 23:26)
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beadbeater24 |
(SynPony @ 22.04.2021 14:47) Добрый день, мы нашли состав буфера для лизиса клеток: DNeasy – like Plant DNA Extraction Protocol (Buffer AP1) 10 mM Tris-HCl pH8.0 1 mM EDTA pH 8.0 0.1% SDS 0.1M NaCl +1X PVP +10mM DTT Скажи пожалуйста, что в данном случае означает 1X PVP Спасибо! обратитесь к Маше логачевой она у нас спец по плантам |
beadbeater24 |
(beadbeater24 @ 27.04.2021 00:35) mlog |
beadbeater24 |
(beadbeater24 @ 27.04.2021 00:37) |
beadbeater24 |
(-Ъ- @ 26.04.2021 23:26) Смело берите 0,5-1,0 % PVP и все будет ОК. По значимости из компонентов буфера PVP на последнем месте. Там SDS работает. если СДС тоды шампунь для жирных волос |
beadbeater24 |
(SynPony @ 22.04.2021 14:47) Добрый день, мы нашли состав буфера для лизиса клеток: DNeasy – like Plant DNA Extraction Protocol (Buffer AP1) 10 mM Tris-HCl pH8.0 1 mM EDTA pH 8.0 0.1% SDS 0.1M NaCl +1X PVP +10mM DTT Скажи пожалуйста, что в данном случае означает 1X PVP Спасибо! позвоните Маше она главная по Геному Гречка |
beadbeater24 |
(beadbeater24 @ 27.04.2021 00:51) Send email Maria Logacheva Assistant Professor Center of Life Sciences Dr. Maria Logacheva is an expert in plant genomics. She graduated from Lomonosov Moscow State University (Biology Department) in 2005. Initially trained as a botanist she then switched to plant genetics and genomics. In 2011 she joined the lab of Alexey Kondrashov and became a key member of the team responsible for the genome analysis using next-generation sequencing. Maria joined Skoltech in 2018 becoming the Head of the Genomics Core Facility. She provided coordination and support of the genomic research programs carried out by Skoltech researchers and participants of Skolkovo ecosystem. Under her supervision GCF became one of the leading objects of scientific infrastructure in Russia. Her research interests focus on plant genomics; currently she is a principal investigator of a project devoted to the identification of genes responsible for economically important traits in buckwheat – of the main agricultural plants in Russia. Another major direction of her studies is the genomics of non-photosynthetic plants, in particular, the patterns of the genome reduction associated with the heterotrophy. Maria published more than 25 articles on different aspects of plant genomics and genetics; she is a winner of several awards, including Moscow Government Award for Young Scientists (2018) and L’Oreal-UNESCO award for women in science (2014). As a member of CLS team Maria plans to continue her research on non-photosynthetic plants expanding its area in a field of functional and comparative genomics, in particular, exploring the response of these plants to light. Also she plans to initiate a new project on the genomics of invasive plants |
beadbeater24 |
(beadbeater24 @ 27.04.2021 00:55) Send email Maria Logacheva Assistant Professor Center of Life Sciences Dr. Maria Logacheva is an expert in plant genomics. She graduated from Lomonosov Moscow State University (Biology Department) in 2005. Initially trained as a botanist she then switched to plant genetics and genomics. In 2011 she joined the lab of Alexey Kondrashov and became a key member of the team responsible for the genome analysis using next-generation sequencing. Maria joined Skoltech in 2018 becoming the Head of the Genomics Core Facility. She provided coordination and support of the genomic research programs carried out by Skoltech researchers and participants of Skolkovo ecosystem. Under her supervision GCF became one of the leading objects of scientific infrastructure in Russia. Her research interests focus on plant genomics; currently she is a principal investigator of a project devoted to the identification of genes responsible for economically important traits in buckwheat – of the main agricultural plants in Russia. Another major direction of her studies is the genomics of non-photosynthetic plants, in particular, the patterns of the genome reduction associated with the heterotrophy. Maria published more than 25 articles on different aspects of plant genomics and genetics; she is a winner of several awards, including Moscow Government Award for Young Scientists (2018) and L’Oreal-UNESCO award for women in science (2014). As a member of CLS team Maria plans to continue her research on non-photosynthetic plants expanding its area in a field of functional and comparative genomics, in particular, exploring the response of these plants to light. Also she plans to initiate a new project on the genomics of invasive plants |
beadbeater24 |
(beadbeater24 @ 27.04.2021 00:51) ну да в Сколтех набирают круглых дур |
beadbeater24 |
(beadbeater24 @ 27.04.2021 00:51) 5:31HD Там шпионки с крепким телом,- Ты их в дверь - они в окно! Говори, что с этим делом Мы покончили давно. Но могут действовать они не прямиком: Шасть в купе - и притвориться мужиком,- А сама наложит тола под корсет. |
SynPony |
(-Ъ- @ 26.04.2021 23:26) Смело берите 0,5-1,0 % PVP и все будет ОК. По значимости из компонентов буфера PVP на последнем месте. Там SDS работает. Спасибо |
SynPony |
(beadbeater24 @ 27.04.2021 01:12) 5:31HD Там шпионки с крепким телом,- Ты их в дверь - они в окно! Говори, что с этим делом Мы покончили давно. Но могут действовать они не прямиком: Шасть в купе - и притвориться мужиком,- А сама наложит тола под корсет. Что? |
watchesbiz Постоянный участник |
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