Molbiol.ru | О проекте | Справочник | Методы | Растворы | Расчёты | Литература | Орг.вопросы Web | Фирмы | Coffee break | Картинки | Работы и услуги | Биржа труда | Zbio-wiki NG SEQUENCING · ЖИЗНЬ РАСТЕНИЙ · БИОХИМИЯ · ГОРОДСКИЕ КОМАРЫ · А.А.ЛЮБИЩЕВ · ЗООМУЗЕЙ Темы за 24 часа [ Вход* | Регистрация* ] Форум: | |
Redactor admin. Берлин, Германия |
Sambrook J., Russell D. 2001; 2344стр.; ISBN: 0879695773 Djvu, 600 dpi, OCR, 32.6 Mb The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three-volume work is essential for everyone using today’s biomolecular techniques. The opening chapters describe essential techniques, some well-established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.
|
|||
Приведённые здесь книги бродит по рукам в электронном виде. На тему о том, "где их взять": 1) прямые ссылки могут быть в комментариях к описанию книги; 2) можно поискать в известных онлайн библиотеках; 3) если первые два способа не помогают, можно 'попросить у коллег' (требуется регистрация!) NB! запросы на книги, которых в каталоге нет, можно делать только в теме "Книги которых нет, но хочется"; из всех других мест запросы удаляются без предупреждений! |
guest: www гость |
_tp://rapidshare.com/files/14860436/0879695765.rar.html слово molbiol ---
|
CellTroll |
Не могли бы Вы перезалить эту книгу, ссылка на скачивание больше не работает. |
*** гость |
лучшее качество кажись у djvu-шек по 10-11 мб
|
Sergeant moderator |
Michael R. Green (Author), Joseph Sambrook (Author) Paperback: 2000 pages Publisher: Cold Spring Harbor Laboratory Press; 4th edition (June 15, 2012) Language: English ISBN-10: 1936113422 ISBN-13: 978-1936113422 Product Dimensions: 11.2 x 9.2 x 4.4 inches THE READY AVAILABILITY OF THE COMPLETE GENOME SEQUENCES for humans and model organisms has profoundly affected the way in which biologists of every discipline now practice science. Exploration of the vast genomic landscape required the development of a great variety of new experimental techniques and approaches. Inevitably, venerable cloning manuals became outdated and established methods were rendered obsolete. These events provided the major impetus for a thorough revision of Molecular Cloning. In the first stages of Molecular Cloning 4 (MC4), we undertook an extensive review process to determine what old material should be kept, what new material should be added, and, most difficult of all, what material should be deleted. The names of the many scientists who contributed invaluable advice during the review process are listed on the following acknowledgments page. We are grateful to them all. It is of course impossible to include in any single laboratory manual all experimental methods used in molecular biology, and so choices, sometimes tough ones, had to be made. Individuals may disagree with some of our choices (and we suspect some will). However, our two guiding principles are as follows. First, Molecular Cloning is a "nucleic acid-centric" laboratory manual, and therefore in general we have not included methods that do not directly involve DNA or RNA. So, although MC4 contains a chapter on yeast two-hybrid procedures for analyzing protein-protein interactions, we did not include the many other approaches for studying protein-protein interactions that do not directly involve nucleic acids. Second, we have attempted to include as many as possible of those nucleic acid-based methods that are widely used in molecular and cellular biology laboratories with the hope in the John Lockean sense that we "do the greatest good for the most people." The harder task for us was to decide which material to delete. However, our task was made somewhat easier by the agreement of our publisher, Cold Spring Harbor Laboratory Press, to make these older methods freely available on the Cold Spring Harbor Protocols website (www.cshprotocols.org). The explosion of new experimental approaches has made it impractical, if not impossible, for any single person (or even two) to write with authority on all the relevant experimental methods. As a result, a major departure from previous editions of Molecular Cloning was the commissioning of experts in the field to write specific chapters and to contribute specific protocols. Without the enthusiastic participation of these scientists, MC4 simply would not exist. Since the last edition of Molecular Cloning, there has been a relentless and continuing proliferation of commercial "kits," which is both a blessing and a curse. On the one hand, kits offer tremendous convenience, particularly for procedures that are not routinely used in an individual laboratory. On the other hand, kits can often be too convenient, enabling users to perform procedures without understanding the underlying principles of the method. Where possible, we have attempted to deal with this dilemma by providing lists of commercially available kits and also describing how they work. There are many people who played essential roles in the production of MC4 and whom we gratefully acknowledge. Djvu OCR 600dpi За ссылками в личку на условии дальнейшего не распостранения книги. Информация о наличии ошибок оцифровки приветствуется.
|
Manu гость |
|
dk Постоянный участник Россия |
|
« Предыдущая тема · Книги · Следующая тема » |