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Mrakocat

21.11.2005 15:16

URL #1

Пожалуйста, помогите найти информацию о СПЭВ. Меня интерисует применение, хранения и использование этой клеточной культуры.

CellCulturist
Постоянный участник
Moscow

25.11.2005 18:04

URL #2

http://www.ecacc.org.uk/

CowDoc
Постоянный участник
Middle East

25.11.2005 18:23

URL #3

ЖИВОТНАЯ КЛЕТКА В КУЛЬТУРЕ (Методы и применение в биотехнологии)
Под общей редакцией
Заслуженного деятеля науки РФ, профессора Л.П. Дьяконова,
Заслуженного ветеринарного врача РФ, профессора В.И. Ситькова
Москва 2000

"Линия клеток почки эмбриона свиньи (СПЭВ)
Перевиваемая линия клеток СПЭВ получена в 1959г. К.С.Куликовой и др. в Московском научно-исследовательском институте вирусных препаратов.
Клетки выращивают на среде 199 с 10% сыворотки крупного рогатого скота. Сплошной монослой образуется через 3-4 дня после посева. Коэффициент посева культур 1:4 - 1:6. Для снятия клеток со стекла используют 0,02%-ый раствор версена. Культура снимается бесцентрифужным методом. Время контакта с клетками при 37 С -10 - 20 мин. Легкое покачивание культурального флакона ускоряет процесс версенизации. Версенизацию проводят до тех пор, пока при микроскопическом исследовании (об. 10, ок.7) не будет обнаружено, что большая часть клеток отслоилась.
Культура представлена эпителиоподобными клетками. Цитоплазма клеток в основном гомогенная, иногда мелко вакуолизирована.
В цитоплазме около ядра отдельных клеток встречаются эозинофильные образования. Ядра клеток округлой или овальной формы с 2-5 ядрышками. Культура пересевается 2 раза в неделю. Если культура выращивается 6-7 дней, то при этом питательная среда слегка мутнеет за счет отслоения клеток. Мутность среды повышается при удлинении сроков инкубации с одновременным изменением рН среды в кислую сторону. Во всех подозрительных случаях необходим бактериологический контроль для выяснения причин помутнения.
Клетки, снятые с одного матраса Ру, можно высеять в 200 пробирок или 4-6 аналогичных матраса. Сбор клеток с одного литрового матраса достигает в среднем 35-40 млн..."

Вообщето это ... ну очень далеко от практики. Морозить совершенно так же как любую другую перевиваемую культуру. Очень кандовые клетки. Вируски растут как свинские, так и КРС (ящурок, чума крс), и многое др.

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URL #4

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URL #5

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URL #10

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