генная инженерия (электрофорез)

скажите пожалуйста почему на электрофорезе после расщепления рестриктазой ЕкоР1 наблюдается 4 полосы , а не 2???

и наоборот 2 на электрофорезе, а не 4??? подробней, думаю связано с формами плазмиды, плазмидной трансформацией и инферсией???
заранее благодарен))

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