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Вебинар будет посвящен работе с первичными культурами гепатоцитов человека (HPHEP).
Клетки HPHEP являются золотым стандартом для исследований в различных областях - in vitro оценке метаболизма лекарственных веществ, при безопасном подборе фармацевтических субстанций, взаимодействии лекарств между собой и моделировании заболеваний. Однако, главным недостатком клеток HPHEP является быстрая потеря клетками своих функций при культивировании в 2D формате, что значительно снижает их пользу.
Компания Takara BIO разработали питательную среду Cellartis® PowerTM Primary HEP Medium для длительного культивирования клеток HPHEP в удобном формате 2D. Среда поддерживает здоровую, функционирующую первичную культуру гепатоцитов человека в течение четырех недель без необходимости использования 3D или сэндвичных культур, позволяя исправить основной недостаток клеток HPHEP.
Dr. Barbara Kuppers-Munther расскажет о культивировании первичных гепатоцитов человека в среде Cellartis® PowerTM Primary HEP Medium, а именно:
- Поддержании жизнеспособности и типичной морфологии клеток гепатоцитов человека,
- Возможности сохранить стабильную секрецию альбумина, - Демонстрация стабильной активности CYP и индукции экспрессии CYP в процессе долгосрочного культивирования.
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