Расчёты и преобразования

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Программы, облегчающие биологической или химические лабораторные расчёты. Конверсия единиц измерения. Операции с нуклеотидной последовательностью: перевод в комплементарную, представление в виде двухцепочечной, трансляция, поиск редких кодонов. Расчёт параметров PCR. Спектрофотометрические измерения. Пересчёт скорости вращения в ускорение. Вычисление времени роста бактерий. Конверсия: масса - моли. Пересчёт молярного количества в молярную концентрацию.



Предлагаю пользователям molbiol на рассмотрение созданную мною утилиту для рассчета параметров олигонуклеотидов.
Утилиту можно найти здесь - OligoCalculator v1.0 ( http://andrew3d.net/index.php?page_id=3005 ).

Привествуются комментарии и замечания по улучшению утилиты.



>gi|22538412|ref|NM_148899.1| Homo sapiens forkhead box P2 (FOXP2), transcript variant 3, mRNA
AGTGAGCTAGCTTCTGAGTTTTCCCTTCTTTTTATACTGTTTTCTGTGCTGGCTTTTTTGAATCTTCCTA
ATTTTTCATCTCTTTAACAAACTCCTATGAAGTTGAAACCGGGAAGTTTGCTCTAACATTTCCAGAGAAG
GTATTAAGTCATGATGCAGGAATCTGCGACAGAGACAATAAGCAACAGTTCAATGAATCAAAATGGAATG
AGCACTCTAAGCAGCCAATTAGATGCTGGCAGCAGAGATGGAAGATCAAGTGGTGACACCAGCTCTGAAG
TAAGCACAGTAGAACTGCTGCATCTGCAACAACAGCAGGCTCTCCAGGCAGCAAGACAACTTCTTTTACA
GCAGCAAACAAGTGGATTGAAATCTCCTAAGAGCAGTGATAAACAGAGACCACTGCAGCAGCCATGAGAC
AGACTTGTGGTTCTTCAACAGCTGTGCAGAGCAGAGACGTAAATTTAAGGTGTGCCTGTGTCAGTGGCCA
TGATGACTCCCCAGGTGATCACCCCTCAGCAAATGCAGCAGATCCTTCAGCAACAAGTCCTGTCTCCTCA
GCAGCTACAAGCCCTTCTCCAACAACAGCAGGCTGTCATGCTGCAGCAGCAACAACTACAAGAGTTTTAC
AAGAAACAGCAAGAGCAGTTACATCTTCAGCTTTTGCAGCAGCAGCAGCAACAGCAGCAGCAGCAACAAC
AGCAGCAACAACAGCAGCAGCAACAACAACAACAACAGCAGCAACAACAGCAGCAGCAGCAGCAACAGCA
GCAGCAGCAGCAACAGCATCCTGGAAAGCAAGCGAAAGAGCAGCAGCAGCAGCAGCAGCAGCAACAGCAA
TTGGCAGCCCAGCAGCTTGTCTTCCAGCAGCAGCTTCTCCAGATGCAACAACTCCAGCAGCAGCAGCATC
TGCTCAGCCTTCAGCGTCAGGGACTCATCTCCATTCCACCTGGCCAGGCAGCACTTCCTGTCCAATCGCT
GCCTCAAGCTGGCTTAAGTCCTGCTGAGATTCAGCAGTTATGGAAAGAAGTGACTGGAGTTCACAGTATG
GAAGACAATGGCATTAAACATGGAGGGCTAGACCTCACTACTAACAATTCCTCCTCGACTACCTCCTCCA
ACACTTCCAAAGCATCACCACCAATAACTCATCATTCCATAGTGAATGGACAGTCTTCAGTTCTAAGTGC
AAGACGAGACAGCTCGTCACATGAGGAGACTGGGGCCTCTCACACTCTCTATGGCCATGGAGTTTGCAAA
TGGCCAGGCTGTGAAAGCATTTGTGAAGATTTTGGACAGTTTTTAAAGCACCTTAACAATGAACACGCAT
TGGATGACCGAAGCACTGCTCAGTGTCGAGTGCAAATGCAGGTGGTGCAACAGTTAGAAATACAGCTTTC
TAAAGAACGCGAACGTCTTCAAGCAATGATGACCCACTTGCACATGCGACCCTCAGAGCCCAAACCATCT
CCCAAACCTCTAAATCTGGTGTCTAGTGTCACCATGTCGAAGAATATGTTGGAGACATCCCCACAGAGCT
TACCTCAAACCCCTACCACACCAACGGCCCCAGTCACCCCGATTACCCAGGGACCCTCAGTAATCACCCC
AGCCAGTGTGCCCAATGTGGGAGCCATACGAAGGCGACATTCAGACAAATACAACATTCCCATGTCATCA
GAAATTGCCCCAAACTATGAATTTTATAAAAATGCAGATGTCAGACCTCCATTTACTTATGCAACTCTCA
TAAGGCAGGCTATCATGGAGTCATCTGACAGGCAGTTAACACTTAATGAAATTTACAGCTGGTTTACACG
GACATTTGCTTACTTCAGGCGTAATGCAGCAACTTGGAAGAATGCAGTACGTCATAATCTTAGCCTGCAC
AAGTGTTTTGTTCGAGTAGAAAATGTTAAAGGAGCAGTATGGACTGTGGATGAAGTAGAATACCAGAAGC
GAAGGTCACAAAAGATAACAGGAAGTCCAACCTTAGTAAAAAATATACCTACCAGTTTAGGCTATGGAGC
AGCTCTTAATGCCAGTTTGCAGGCTGCCTTGGCAGAGAGCAGTTTACCTTTGCTAAGTAATCCTGGACTG
ATAAATAATGCATCCAGTGGCCTACTGCAGGCCGTCCACGAAGACCTCAATGGTTCTCTGGATCACATTG
ACAGCAATGGAAACAGTAGTCCGGGCTGCTCACCTCAGCCGCACATACATTCAATCCACGTCAAGGAAGA
GCCAGTGATTGCAGAGGATGAAGACTGCCCAATGTCCTTAGTGACAACAGCTAATCACAGTCCAGAATTA
GAAGACGACAGAGAGATTGAAGAAGAGCCTTTATCTGAAGATCTGGAATGAGAACTGACTTGTGAAACCT
CAGCGTGAAGGGACATATCACTGACCTTCATAACCACTCCACAACCATGAATATTTGACAAATTTTTACT
GTGACTATTTATTAAGCATGGATAAAGGAGACAGCCCTAAAGGAACTTACTAAGCCAGCCCTTTGGGATT
CAGTACCAACAGGCA

>gi|57113920|ref|NM_001009020.1| Pan troglodytes forkhead box P2 (FOXP2), mRNA
TGAGCTAGCTTCTGAGTTTTCCCTTCTTTTTATACTGTTTTCTGCGCTGGCTTTTTTGAATCTTCCTAAT
TTTTCATCTCTTTAACAAACTCCTATGAAGTTGAAACCGGGAAGTTTGCTCTAACATTTCCAGAGAAGGT
ATTAAGTCATGATGCAGGAATCTGCGACAGAGACAATAAGCAACAGTTCAATGAATCAAAATGGAATGAG
CACTCTAAGCAGCCAATTAGATGCTGGCAGCAGAGATGGAAGATCAAGTGGTGACACCAGCTCTGAAGTA
AGCACAGTAGAACTGCTGCATCTGCAACAACAGCAGGCTCTCCAGGCGGCAAGACAACTTCTTTTACAGC
AGCAAACAAGTGGATTGAAATCTCCTAAGAGCAGTGATAAACAGAGACCACTGCAGGTGCCTGTGTCAGT
GGCCATGATGACTCCCCAGGTGATCACCCCTCAGCAAATGCAGCAGATCCTTCAGCAACAAGTCCTGTCT
CCTCAGCAGCTCCAAGCCCTTCTCCAACAACAGCAGGCTGTCATGCTGCAGCAGCAACAACTACAAGAGT
TTTACAAGAAACAGCAAGAGCAGTTACATCTTCAGCTTTTGCAGCAGCAGCAGCAACAGCAGCAGCAGCA
ACAACAGCAGCAACAACAGCAGCAGCAACAACAACAACAGCAGCAGCAGCAACAACAGCAGCAGCAGCAG
CAACAGCAGCAGCAGCAGCAACAGCATCCTGGAAAGCAAGCGAAAGAGCAGCAGCAGCAGCAGCAGCAGC
AACAGCAATTGGCAGCCCAGCAGCTTGTCTTCCAGCAGCAGCTTCTCCAGATGCAACAACTCCAGCAGCA
GCAGCATCTGCTCAGCCTTCAGCGTCAGGGACTCATCTCCATTCCACCTGGCCAGGCAGCACTTCCTGTC
CAATCGCTGCCTCAAGCTGGCTTAAGTCCTGCTGAGATTCAGCAGTTATGGAAAGAAGTGACTGGAGTTC
ACAGTATGGAAGACAATGGCATTAAACATGGAGGGCTAGACCTCACTACTAACAATTCCTCCTCGACTAC
CTCCTCCACCACTTCCAAAGCGTCACCACCAATAACTCATCATTCCATCGTGAATGGACAGTCTTCAGTT
CTAAATGCAAGACGAGACAGCTCGTCACATGAGGAGACTGGGGCCTCTCACACTCTCTATGGCCATGGAG
TTTGCAAATGGCCAGGCTGTGAAAGCATTTGTGAAGATTTTGGACAGTTTTTAAAGCACCTTAACAATGA
ACACGCATTGGATGACCGAAGCACTGCTCAGTGTCGAGTGCAAATGCAGGTGGTGCAACAGTTAGAAATA
CAGCTTTCTAAAGAACGCGAACGTCTTCAAGCAATGATGACCCACTTGCACATGCGACCCTCAGAGCCCA
AACCATCTCCCAAACCTCTAAATCTGGTGTCTAGTGTCACCATGTCGAAGAATATGTTGGAGACATCCCC
ACAGAGCTTACCTCAAACCCCTACCACACCAACGGCCCCAGTCACCCCGATTACCCAGGGACCCTCAGTA
ATCACCCCAGCCAGTGTGCCCAATGTGGGAGCCATACGAAGGCGACATTCAGACAAATACAACATTCCCA
TGTCATCAGAAATTGCCCCAAACTATGAATTTTATAAAAATGCAGATGTCAGACCTCCATTTACTTATGC
AACTCTCATAAGGCAGGCTATCATGGAGTCATCTGACAGGCAGTTAACACTTAATGAAATTTACAGCTGG
TTTACACGGACATTTGCTTACTTCAGGCGTAATGCAGCAACTTGGAAGAATGCAGTACGTCATAATCTTA
GCCTGCACAAGTGTTTTGTTCGAGTAGAAAATGTTAAAGGAGCAGTATGGACTGTGGATGAAGTAGAATA
CCAGAAGCGAAGGTCACAAAAGATAACAGGAAGTCCAACCTTAGTAAAAAATATACCTACCAGTTTAGGC
TATGGAGCAGCTCTTAATGCCAGTTTGCAGGCTGCCTTGGCAGAGAGCAGTTTACCTTTGCTAAGTAATC
CTGGACTGATAAATAATGCATCCAGTGGCCTACTGCAGGCCGTCCACGAAGACCTCAATGGTTCTCTGGA
TCACATCGACAGCAATGGAAACAGTAGTCCGGGCTGCTCACCTCAGCCGCACATACATTCAATCCACGTC
AAGGAAGAGCCAGTGATTGCAGAGGATGAAGACTGCCCAATGTCCTTAGTGACAACAGCTAATCACAGTC
CAGAATTAGAAGACGACAGAGAGATTGAAGAAGAGCCTTTATCTGAAGATCTGGAATGAGAACTGACTTG
TGAAACCTCAGCGTGAAGGGACATATCACTGACCTTCATAACCACTCCACAACCATGAATATTTGACAAA
TTTTTACTGTGACTATTTATTAAGCATGGATAAAGGAGACAGCCCTAAAGGAACTTACTAAGCCAGCCCT
TTGGGATTCA



gi|22538412|ref|NM_148899.1| Homo sapiens forkhead box P2 (FOXP2), transcript variant 3, mRNA
AGTGAGCTAGCTTCTGAGTTTTCCCTTCTTTTTATACTGTTTTCTGTGCTGGCTTTTTTGAATCTTCCTA
ATTTTTCATCTCTTTAACAAACTCCTATGAAGTTGAAACCGGGAAGTTTGCTCTAACATTTCCAGAGAAG
GTATTAAGTCATGATGCAGGAATCTGCGACAGAGACAATAAGCAACAGTTCAATGAATCAAAATGGAATG
AGCACTCTAAGCAGCCAATTAGATGCTGGCAGCAGAGATGGAAGATCAAGTGGTGACACCAGCTCTGAAG
TAAGCACAGTAGAACTGCTGCATCTGCAACAACAGCAGGCTCTCCAGGCAGCAAGACAACTTCTTTTACA
GCAGCAAACAAGTGGATTGAAATCTCCTAAGAGCAGTGATAAACAGAGACCACTGCAGCAGCCATGAGAC
AGACTTGTGGTTCTTCAACAGCTGTGCAGAGCAGAGACGTAAATTTAAGGTGTGCCTGTGTCAGTGGCCA
TGATGACTCCCCAGGTGATCACCCCTCAGCAAATGCAGCAGATCCTTCAGCAACAAGTCCTGTCTCCTCA
GCAGCTACAAGCCCTTCTCCAACAACAGCAGGCTGTCATGCTGCAGCAGCAACAACTACAAGAGTTTTAC
AAGAAACAGCAAGAGCAGTTACATCTTCAGCTTTTGCAGCAGCAGCAGCAACAGCAGCAGCAGCAACAAC

>gi|57113920|ref|NM_001009020.1| Pan troglodytes forkhead box P2 (FOXP2), mRNA
TGAGCTAGCTTCTGAGTTTTCCCTTCTTTTTATACTGTTTTCTGCGCTGGCTTTTTTGAATCTTCCTAAT
TTTTCATCTCTTTAACAAACTCCTATGAAGTTGAAACCGGGAAGTTTGCTCTAACATTTCCAGAGAAGGT
ATTAAGTCATGATGCAGGAATCTGCGACAGAGACAATAAGCAACAGTTCAATGAATCAAAATGGAATGAG
CACTCTAAGCAGCCAATTAGATGCTGGCAGCAGAGATGGAAGATCAAGTGGTGACACCAGCTCTGAAGTA
AGCACAGTAGAACTGCTGCATCTGCAACAACAGCAGGCTCTCCAGGCGGCAAGACAACTTCTTTTACAGC
AGCAAACAAGTGGATTGAAATCTCCTAAGAGCAGTGATAAACAGAGACCACTGCAGGTGCCTGTGTCAGT
GGCCATGATGACTCCCCAGGTGATCACCCCTCAGCAAATGCAGCAGATCCTTCAGCAACAAGTCCTGTCT
CCTCAGCAGCTCCAAGCCCTTCTCCAACAACAGCAGGCTGTCATGCTGCAGCAGCAACAACTACAAGAGT
TTTACAAGAAACAGCAAGAGCAGTTACATCTTCAGCTTTTGCAGCAGCAGCAGCAACAGCAGCAGCAGCA
ACAACAGCAGCAACAACAGCAGCAGCAACAACAACAACAGCAGCAGCAGCAACAACAGCAGCAGCAGCAG
CAACAGCAGCAGCAGCAGCAACAGCATCCTGGAAAGCAAGCGAAAGAGCAGCAGCAGCAGCAGCAGCAGC
AACAGCAATTGGCAGCCCAGCAGCTTGTCTTCCAGCAGCAGCTTCTCCAGATGCAACAACTCCAGCAGCA
GCAGCATCTGCTCAGCCTTCAGCGTCAGGGACTCATCTCCATTCCACCTGGCCAGGCAGCACTTCCTGTC
CAATCGCTGCCTCAAGCTGGCTTAAGTCCTGCTGAGATTCAGCAGTTATGGAAAGAAGTGACTGGAGTTC
ACAGTATGGAAGACAATGGCATTAAACATGGAGGGCTAGACCTCACTACTAACAATTCCTCCTCGACTAC
CTCCTCCACCACTTCCAAAGCGTCACCACCAATAACTCATCATTCCATCGTGAATGGACAGTCTTCAGTT
CTAAATGCAAGACGAGACAGCTCGTCACATGAGGAGACTGGGGCCTCTCACACTCTCTATGGCCATGGAG
TTTGCAAATGGCCAGGCTGTGAAAGCATTTGTGAAGATTTTGGACAGTTTTTAAAGCACCTTAACAATGA




"Гранату в рот- и выдернуть чеку"



Уважаемые,
есть ли у кого-нибудь калькулятор еффективности ФРЕТ? Например, при известном изменении расстояния между парой донор/акцептор (и известном Фёрстр-радиусе)? А также "наоборот": вычисление расстояния при известной эффективности ФРЕТ?



http://translate.google.ru/translate?hl=ru...ochemistry/fret ( http://translate.google.ru/translate?hl=ru&langpair=en|ru&u=http://www.calctool.org/CALC/chem/photochemistry/fret )



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