You want to determine if an individual is homozygous for the normal allele, homozygous for the sickle-cell allele, or heterozygous for alleles of the β-globin gene. From three individuals from the same family, you PCR amplified a 1000 base pair segment of DNA centered on the point mutation that encodes the sixth amino acid in β-globin. Using these PCR products, design an experiment using the restriction enzyme that you selected in part D to test for the presence or absence of this mutation. Drag and drop the bands onto the DNA gel below to represent the results for the restriction digest of the 1000 base pair PCR product using the enzyme that you have chosen in part D. When you drag and drop the bands, only use a band to indicate the length of a fragment and not the quantity.

In lane 1, give the pattern expected for an individual that is homozygous for the normal allele of the gene encoding β-globin by dragging and dropping bands onto the appropriate position.

In lane 2, give the pattern expected for an individual that is heterozygous for the gene encoding β-globin by dragging and dropping bands onto the appropriate position.

In lane 3, give the pattern expected for an individual that is homozygous for the sickle-cell allele of the gene encoding β-globin by dragging and dropping bands onto the appropriate position. The DNA ladder is given. Be sure to align the center of the band with the light vertical lines.

  Я надеялся здесь увидеть растерзанное тело. Ан нет. Странно.

  Ищите в поиске по нуцлеотиде на хуман бета-глобин. Потом в домашке не забудьте указать аццессион нумбер последовательности, ваш профессор будет впечатлен )

  Ищите в поиске по nucleotide на human beta-globin. Потом в домашке не забудьте указать accession number последовательности, ваш профессор будет впечатлен )