Как измерить молекулярный вес белкового комплекса, более

?



Гельфильтрацией, предварительно откалибровав колонку.



Superose6 колонка от Pharmacia-Amersham
Маркеры мол веса от Sigma.
Можно легко отделить 2MD от 1MD
Удачи



Не мучайтесь вы с гельфильтрацией, ничего путного все равно не измерите.
Найдите лучше людей с аналитической ультрацентрифугой.



Спасибо за советы.
Хотел бы узнать более точно.
Что за маркеры от Sigma? - у нас есть самое большое - 670 кДа, да и то деградированный
Есть ли в природе большие?
Все-таки мне кажется, что при весе примерно несколько миллионов Да - седиментация лучше, чем гель-фильтрация



В сигмовских маркерах в сопроводиловке написано, что там такое. Главное - не перепутать форезные с хроматографическими.)) Если нужны большие, то можно пошить глутаровым альдегидом, предварительно подобрав условия. Получается очень даже неплохо.



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