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ўЄлкните, чтобы внести в »збранные “емы* √ен UPF1 мутирует при аденоскваматозной карциноме поджелудочной железы

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 прочитанное сообщение 16.07.2014 18:12     —ообщение дл€ модератора         Ћичное письмо  ќтправить e-mail

картинка: UPF1.png¬ опухол€х аденоскваматозной карциномы поджелудочной железы обнаружены мутации гена UPF1, инактивирующие комплекс деградации неправильно сплайсированных м–Ќ . ƒефект этого комплекса в случае раковых опухолей показан впервые. ƒетекци€ мутаций может позволить усовершенствовать диагностику аденоскваматозной карциномы, а субстраты комплекса в принципе могут стать мишен€ми дл€ разработки новых методов терапии рака.

јденоскваматозна€ карцинома поджелудочной железы (ј— ) Ц чрезвычайно агрессивна€ и часто метастазирующа€ опухоль, превосход€ща€ по этим свойствам даже аденокарциному. ≈е генетика была слабо изучена. ¬ опухол€х ј—  были найдены мутации генов KRAS и TP53, свойственные опухол€м других форм рака поджелудочной железы, но никаких мутаций, специфических дл€ ј— .

јвторы обнаружили, что в опухол€х ј—  с аллелем “–53 дикого типа присутствует м–Ќ  р53 аномального размера, отсутствующа€ в нормальной ткани. —еквенирование показало, что она представл€ет собой продукт альтернативного сплайсинга с использованием неканонических донора сплайсинга в интроне 6 и акцептора в интроне 10. ¬ результате по€вл€лс€ терминирующий кодон, прерывающий синтез р53. јномальна€ м–Ќ  обнаруживалась только в опухоли, но не в окружающей ткани, а нормальна€ м–Ќ  р53 в обеих ткан€х. ¬ норме м–Ќ  с кодонами досрочной терминации трансл€ции деградируютс€ комплексом NMD (nonsense- mediated RNA decay). јвторы предположили, что в опухол€х ј—  нарушен этот комплекс. ѕр€мое секвенирование показало, что в опухол€х 18 из 23 больных ј—  имеютс€ мутации в гене UPF1. ¬ других генах комплекса NMD Ц UPF2, UPF3ј, UPF3¬ - мутаций не найдено. ћутации UPF1 не обнаружены в не-ј—  панкреатических опухол€х и в опухол€х скваматозной карциномы легких.

¬се найденные мутации сосредоточены в экзонах и интронах двух областей гена UPF1. Ћокализаци€ мутаций позволила предположить, что многие из них наруша€ структуру экзонных и интронных энхансеров сплайсинга блокируют нормальный сплайсинг м–Ќ  UPF1. ƒл€ проверки предположени€ были сконструированы мини-гены, содержащие последовательности обоих мутирующих участков UPF1. ¬ведение в них мутаций, найденных у 15 пациентов, приводило к аномальному сплайсингу м–Ќ  в соотношении 10:1 и более по сравнению с нормальным сплайсингом. ѕри этом элиминировались части одного из двух доменов, необходимых дл€ нормального функционировани€ UPF1.

¬ соответствии с этими результатами показано, что в опухол€х ј— , в отличие от окружающей ткани, снижено содержание или вообще отсутствует белок UPF1 и накапливаютс€ субстраты NMD. ¬ случае ј—  дефект NMD сдвигает баланс между нормально и аномально сплайсированными м–Ќ , что, веро€тно, приводит к усилению малигнизации. ќбнаружение в опухол€х ј—  мутаций в комплексе NMD впервые показало участие дефектов NMD в онкогенезе. ƒетекци€ этих мутаций может позволить усовершенствовать диагностику ј— , а субстраты NMD в принципе могут стать мишен€ми дл€ разработки новых методов терапии рака.


»сточник: Liu C, Karam R, Zhou Y, Su F, Ji Y, Li G, Xu G, Lu L, Wang C, Song M, Zhu J, Wang Y, Zhao Y, Foo WC, Zuo M, Valasek MA, Javle M, Wilkinson MF, Lu Y. The UPF1 RNA surveillance gene is commonly mutated in pancreatic adenosquamous carcinoma. // Nat Med. 2014; V. 20: P. 596-598.

ѕодпись к рисунку: Ћокализаци€ мутаций, обнаруженных в гене UPF1 при аденоскваматозной карциноме поджелудочной железы.

¬сего благодарностей: 2ѕоблагодарили (2): Danse, Vadim Sharov



—ообщение в колонке новостей: Ќаука: обща€ и молекул€рна€ биологи€—ообщение в колонке новостей, раздел "Ќаука: обща€ и молекул€рна€ биологи€"
29.07.2014 09:27
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 прочитанное сообщение 01.08.2014 23:23     —ообщение дл€ модератора         Ћичное письмо  ќтправить e-mail
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ќтлична€ стать€ и очень хорошее summary.

Ќо "аденоскваматозна€ карцинома" - это все-таки уже слишкомsmile.gif

Ћучше просто и по-русски - железисто-плоскоклеточный рак поджелудочной железы.
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 прочитанное сообщение 29.08.2014 14:55     —ообщение дл€ модератора       
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(Telbin @ 01.08.2014 23:23)
—сылка на исходное сообщени円ќтлична€ стать€ и очень хорошее summary.

Ќо "аденоскваматозна€ карцинома" - это все-таки уже слишкомsmile.gif

Ћучше просто и по-русски - железисто-плоскоклеточный рак поджелудочной железы.

http://rrnews.ru/news/u4844/2014/08/29/76114

smile.gif
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 прочитанное сообщение 25.07.2018 11:41     —ообщение дл€ модератора         Ћичное письмо  ќтправить e-mail
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ќтлична€ стать€ и очень хорошее summary.
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 прочитанное сообщение —ообщение на английском  26.07.2018 11:22     —ообщение дл€ модератора         Ћичное письмо  ќтправить e-mail
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 прочитанное сообщение —ообщение на английском  07.08.2018 08:29     —ообщение дл€ модератора         Ћичное письмо  ќтправить e-mail  Web-адрес
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 прочитанное сообщение —ообщение на английском  01.11.2018 18:21     —ообщение дл€ модератора       
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 прочитанное сообщение 16.09.2020 19:50     —ообщение дл€ модератора         Ћичное письмо  ќтправить e-mail
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 прочитанное сообщение —ообщение на английском  19.11.2020 08:45     —ообщение дл€ модератора       
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This was really an interesting topic and I kinda agree with what you have mentioned here!
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I would like to say that this blog really convinced me to do it! Thanks, very good post.

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 прочитанное сообщение —ообщение на английском  03.02.2021 22:55     —ообщение дл€ модератора         Ћичное письмо  ќтправить e-mail
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The name of the popular puzzle is a play on the familiar phrase. It is also a reference to the hexagonal shape of the puzzle. The six-sided body is similar to the form of a honeycomb in a beehive. https://nytimes-spellingbee.com/
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 прочитанное сообщение —ообщение на английском  05.04.2021 09:59     —ообщение дл€ модератора         Ћичное письмо
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Ten Sports Live - Watch ten sports online is a very popular sports channel. It covers sports all over the world. You can watch all kinds of sports like cricket, football, hockey etc. on this channel. You can watch all PSL matches live on this channel. It covers almost all the leagues not only PSL but all over the world.

ten sports live

Ptv Sports Live Streaming - Watch Ptv Sports Online is a sports channel of the Pakistani Government. It broadcasts all sports inside and outside Pakistan. It is owned by TV corporation Pakistan. It was launched in 2012. His slagon is "Jeet hai janoon and hai game." Using pak24tv.com to watch its live streaming.

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 прочитанное сообщение —ообщение на английском  14.05.2021 11:13     —ообщение дл€ модератора         Ћичное письмо  ќтправить e-mail
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 прочитанное сообщение —ообщение на английском  30.05.2021 10:58     —ообщение дл€ модератора       
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ten sports live - Watch ten sports online is a very popular sports channel. It covers sports all over the world.
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 прочитанное сообщение —ообщение на английском  31.05.2021 08:00     —ообщение дл€ модератора         Ћичное письмо  ќтправить e-mail
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 прочитанное сообщение —ообщение на английском  26.07.2021 08:23     —ообщение дл€ модератора         Ћичное письмо
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ptv sports live Streaming - Watch PTV Sports Online is a sports channel of the Pakistani Government.
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 прочитанное сообщение —ообщение на английском  31.07.2021 15:03     —ообщение дл€ модератора         Ћичное письмо  ќтправить e-mail
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”частник оффлайн! ashimagupta02
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 прочитанное сообщение —ообщение на английском  31.07.2021 15:15     —ообщение дл€ модератора         Ћичное письмо  ќтправить e-mail
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 прочитанное сообщение —ообщение на английском  31.07.2021 15:20     —ообщение дл€ модератора         Ћичное письмо  ќтправить e-mail
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