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Как лучше крутить культуру стафилококка, чтобы и осадок был хороший, и клетки не повреждались?

Guest 06.03.2016 19:45
ночная культура S. aureus, сделана на TSB 8 мл + 20ul вчерашней культуры (OD 1.0). Затем по 500 ul культуры в 1,5 ml пробирки, центрифугирование, убрать супернатант, отмывать 3 раза по 500 ul PBS. В конце осадка в 3 раза меньше, супернатант все время прозрачный. Центрифуга Эппендорф. Клетки теряются или осадок становится более компактным?
Вопрос: g?




/ Сборник задач,  #568590  /
Первые решившие: пока никто (из 10 // осталось: 10)
Область: Микробиология (бактериология, вирусология, микология)
Характер и уровень: Простые (расч), Простые (опред)




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The Pirates announced their signing of Shim, a product of Seoul-based Duksoo High School, and 21 other international free agents on Jan. 16. Shim flew to the U.S. over the weekend and visited the Pirates' home park on the cold, snowy day. 먹튀검증 ( https://muksabu.com/ )



totooffice 27.01.2023 16:50
The 18-year-old right-hander had been ranked No. 10 among international prospects by MLB Pipeline. He skipped last year's rookie draft for the Korea Baseball Organization (KBO), where he would have been a high first-round pick, so he could sign with a big league club. 토토사이트 ( https://totoffice.com/ )





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