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tdzeta |
Может кто-то помнит какую-нибудь ссылку и т.д.? Заранее благодарю. |
Esya Постоянный участник PA, USA |
а что они делали при 9.5? это малость экстрим |
tdzeta |
На 9.5 они делали гибриды сефарозы с модельными РНК - точнее, модификацию сефарозы РНКой. |
Esya Постоянный участник PA, USA |
сами поищите RNA stability alkaline conditions |
tdzeta |
Kinetics and mechanisms for the cleavage and isomerization of the phosphodiester bonds of RNA by Bronsted acids and bases By:Oivanen, M (Oivanen, M); Kuusela, S (Kuusela, S); Lonnberg, H (Lonnberg, H) CHEMICAL REVIEWS Volume: 98 Issue: 3 Pages: 961-990 DOI: 10.1021/cr960425x Published: MAY 1998 1. Introduction 2. Specific Acid/Base Catalyzed Cleavage and Isomerization of the 3‘,5‘-Phosphodiester Bonds A. Minimal Reaction Scheme B. The Reactive Ionic Forms C. Hydronium Ion Catalyzed Reactions of Neutral Phosphodiester D. Uncatalyzed Reactions of Neutral Phosphodiester E. Uncatalyzed Reactions of Phosphodiester Monoanion F. Cleavage via a Dianionic Phosphorane Intermediate/Transition State 3. General Acid/Base Catalyzed Cleavage and Isomerization of the 3‘,5‘- Phosphodiester Bonds A. General B. Catalysis by Imidazole and Morpholine Buffers C. Mechanism of the Imidazole/Imidazolium Ion (and Morpholine/Morpholinium Ion) Catalysis D. Catalysis by Carboxylate Buffer 4. Structural Effects A. The Effect of Leaving Group on the Cleavage via a Dianionic Pentacoordinated Transition State B. The Effect of Leaving Group on the Cleavage and Isomerization via a Phosphorane Intermediate C. The Effect of Sugar Moiety Structure D. The Effect of Base Moiety 5. Reactions of 2‘,3‘-Cyclic Phosphates A. Hydrolysis of Nucleoside 2‘,3‘-Cyclic Phosphates B. Interconversion and Dephosphorylation of Nucleoside 2‘- and 3‘-Phosphates 6. The Cleavage and Isomerization of the Phosphodiester Bonds of Polyribonucleotides 7. Cleaving Agents. Toward Artificial Nucleases by Proton-Transfer Catalysis A. Aliphatic Di- and Polyamines as Cleaving Agents B. Cleavage by Peptides C. Imidazole-Based Cleaving Agents D. Bis(guanidinium)-Based Cleaving Agents E. Oligonucleotide Conjugates as Sequence-Selective Cleaving Agents F. Synthetic Polymers as Cleaving Agents G. Cleavage of Five-Membered Cyclic Phosphates by RNase Mimetics 8. The Effect of Thia and Aza Substitutions on the Transesterification of Ribonucleoside 3‘-Phosphodiesters A. Amino and Mercapto Groups as Intramolecular Nucleophiles B. Reactions of 5‘-Phosphorothiolates and 5‘-Phosphoramidates C. Reactions of 3‘-Phosphorothiolates and 3‘-Phosphoramidates D. Reactions of Phosphorothioates and Phosphoramidates Bearing Substitutions in Nonbridging Position 9. References Это основная проблема |
watchesonline89 Постоянный участник |
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