Table of Contents
- 2-D Protein Gel Electrophoresis: An Overview (pp. 1-8)
Fichmann, Jenny; Westermeier, Reiner - Solubilization of Proteins in 2-D Electrophoresis: An Outline (pp. 9-20)
Rabilloud, Thierry - Preparation of Escherichia co/i Samples for 2-D Gel Analysis (pp. 21-30)
VanBogelen, Ruth A.; Neidhardt, Frederick C. - Preparing 2-D Protein Extracts from Yeast (pp. 31-34)
Schieliz, David M. - 2-D Protein Extracts from Drosophila melanogaster (pp. 35-42)
Ericsson, Christer - Preparing 2-D Protein Extracts from Caenorhabditis elegsns (pp. 43-48)
Zwilling, Robert - Eukaryotic Cell Labeling and Preparation for 2-D (pp. 49-52)
Bizios, Nick - Differential Detergent Fractionation of Eukaryotic Cells: Analysis by Two- Dimensional Gel Electrophoresis (pp. 53-66)
Ramsby, Melinda L.; Makowski, Gregory S. - Fractionated Extraction of Total Tissue Proteins from Mouse and Human for 2-D Electrophoresis (pp. 67-86)
Klose, Joachim - Preparation and Solubilization of Body Fluids for 2-D (pp. 87-94)
Sanchez, Jean-Charles; Hochstrasser, Denis F. - D Electrophoresis of Plant Proteins (pp. 95-98)
Tsugita, Akira; Kamo, Masaharu - Quantifying Protein in 2-D PAGE Solubilization Buffers (pp. 99-104)
Ramagli, Louis S. - Measuring the Radioactivity of 2-D Protein Extracts (pp. 105-108)
Link, Andrew J.; Bizios, Nick - Advantages of Carrier Ampholyte IEF (pp. 109-110)
Lopez, Mary F. - 2-D Electrophoresis Using Carrier Ampholytes in the First Dimension (IEF) (pp. 111-128)
Lopez, Mary F. - Nonequilibrium pH Gel Electrophoresis (NEPHGE) (pp. 129-132)
Lopez, Mary F. - High-Resolution, 2-D Protein Electrophoresis Using Nondedicated Equipment (pp. 133-146)
Sarmiento, Marion - Large-Gel 2-D Electrophoresis (pp. 147-172)
Klose, Joachim - Advantages of Immobilized pH Gradients (pp. 173-174)
Fichmann, Jenny - Casting Immobilized pH Gradients (IPGs) (pp. 175-188)
Gianazza, Elisabetta - Analytical IPG-Dalt (pp. 189-196)
Gorg, Angelika; Weiss, Walter - IPG-Dalt of Very Alkaline Proteins (pp. 197-210)
Gorg, Angelika - Running Preparative Carrier Ampholyte and Immobilized pH Gradient IEF Gels for 2-D (pp. 211-220)
Matsui, Neil M.; Smith-Beckerman, Diana M.; Fichmann, Jenny; Epstein, Lois B. - In-Gel Sample Rehydration of Immobilized pH Gradient (pp. 221-226)
Sanchez, Jean-Charles; Hochstrasser, Denis; Rabilloud, Thierry - High-Resolution, IPG-Based, Mini Two-Dimensional Gel Electrophoresis (pp. 227-234)
Sanchez, Jean-Charles; Hochstrasser, Denis F. - Horizontal SDS-PAGE for IPG-Dalt (pp. 235-244)
Gorg, Angelika; Weiss, Walter - Casting and Running Vertical Slab-Gel Electrophoresis for 2D-PAGE (pp. 245-254)
Walsh, Bradley J.; Herbert, Benjamin R. - Nonreducing 2-D Polyacrylamide Gel Electrophoresis (pp. 255-264)
Ji, Hong; Simpson, Richard J. - 2-D Diagonal Gel Electrophoresis (pp. 265-270)
Goverman, Joan - 2-D Phosphopeptide Mapping (pp. 271-280)
Nagahara, Hikaru; Latek, Robert R.; Ezhevsky, Sergei A.; Dowdy, Steven F. - Internal Standards for 2-D (pp. 281-284)
Link, Andrew J. - Autoradiography of 2-D Gels (pp. 285-290)
Link, Andrew J. - Double-Label Analysis (pp. 291-296)
Lee, Kelvin H.; Harrington, Michael G. - Silver Staining of 2-D Electrophoresis Gels (pp. 297-306)
Rabilloud, Thierry - Staining of Preparative 2-D Gels: Coomassie Blue and Imiciazole-Zinc Negative Staining (pp. 307-312)
Matsui, Neil M.; Smith-Beckerman, Diana M.; Epstein, Lois B. - Electroblotting of Proteins from 2-D Polyacrylamide Gels (pp. 313-318)
Dunn, Michael J. - Detection of Total Proteins on Western Blots of 2-D Polyacrylamide Gels (pp. 319-330)
Dunn, Michael J. - Protein Detection Using Reversible Metal Chelate Stains (pp. 331-340)
Patton, Wayne F.; Lim, Mark J.; Shepro, David - Glycoprotein Detection of 2-D Separated Proteins (pp. 341-352)
Packer, Nicolle H.; Ball, Malcolm S.; Devine, Peter L. - Image Acquisition in 2-D Electrophoresis (pp. 353-362)
Patton, Wayne F.; Lim, Mark J.; Shepro, David - Computer Analysis of 2-D Images (pp. 363-382)
Appel, Ron D.; Hochstrasser, Denis F. - D Databases on the World Wide Web (pp. 383-392)
Appel, Ron D.; Bairoch, Amos; Hochstrasser, Denis F. - Comparing 2-D Electrophoretic Gels Across Internet Databases (pp. 393-410)
Lemkin, Peter F. - Constructing a 2-D Database for the World Wide Web (pp. 411-416)
Appel, Ron D.; Hoogland, Christine; Bairoch, Amos; Hochstrasser, Denis F. - Absolute Quantitation of 2-D Protein Spots (pp. 417-422)
Gygi, Steven P.; Aebersold, Ruedi - Generating a Bacterial Genome Inventory: Identifying 2-D Spots by Comigrating Products of the Genome on 2-D Gels (pp. 423-430)
VanBogelen, Ruth A. - lmmunoaffinity Identification of 2-DE Separated Proteins (pp. 431-444)
Magi, Barbara; Bini, Luca; Marzocchi, Barbara; Liberatori, Sabrina; Raggiaschi, Roberto; Pallini, Vitaliano - 2-DE Spot Amino Acid Analysis with 9-Fluorenylmethyl Chloroformate (pp. 445-460)
Wilkins, Marc R.; Yan, Jun X.; Gooley, Andrew A. - N-Terminal Amino Acid Sequencing of 2-DE Spots (pp. 461-466)
Kamo, Masaharu; Tsugita, Akira - Characterizing Proteins from 2-DE Gels by Internal Sequence Analysis of Peptide Fragments: Strategies for Microsample Handling (pp. 467-472)
Erdjument-Bromage, Hediye; Lui, Mary; Lacomis, Lynne; Tempst, Paul - Obtaining Molecular Weights of Proteins and Their Cleavage Products by Directly Combining Gel Electrophoresis with Mass Spectrometry (pp. 473-486)
Loo, Rachel R. Ogorzalek; Loo, Joseph A.; Andrews, Philip C. - Identification of Proteins by Matrix-Assisted Laser DesorptiorVlonization Mass Spectrometry Using Peptide and Fragment Ion Masses (pp. 487-512)
Courchesne, Paul L.; Pattersonv, Scott D. - Sample Preparation Methods for Mass Spectrometric Peptide Mapping Directly from 2-DE Gels (pp. 513-530)
Jensen, Ole Norrregaard; Wilm, Matthias; Shevchenko, Andrej; Mann, Matthias - Protein Identification and Analysis Tools in the ExPASy Server (pp. 531-552)
Wilkins, Marc R.; Gasteiger, Elisabeth; Bairoch, Amos; Sanchez, Jean-Charles; Williams, Keith L.; Appel, Ron D.; Hochstrasser, Denis F. - Automated Protein Identification Using Microcolumn Liquid Chromatography-Tandem Mass Spectrometry (pp. 553-570)
Yates, John R.; Carmack, Edwin; Hays, Lara; Link, Andrew J.; Eng, Jimmy K. - Peptide Sequencing of 2-DE Gel-Isolated Proteins by Nanoelectrospray Tandem Mass Spectrometry (pp. 571-588)
Jensen, Ole Norregaard; Wilm, Matthias; Shevchenko, Andrej; Mann, Matthias
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