AcO^-

AcONH₄

Mw=77.08g/M; (lagern bei NT).

Konz. Stamm. %(w/w) 10ml 50ml 100ml 150ml CH3COONH4 10M 77.08g/M 71.84 7.708g 38.54g 77.08g 115.62g H2O   mQ 28.16 3.022g 15.11g 30.22g 45.33g

10M: p=1.073.

Konz. Stamm. 50ml 100ml 200ml AcONH4 7.5M 10M 37.5ml 40.24g 75ml 80.48g 150ml 160.95g H2O   mQ 12.5ml 25ml 50ml

Konz. Stamm. 50ml 100ml 200ml AcONH4 2.0M 10M 10ml 10.73g 20ml 21.46g 40ml 42.92g H2O   mQ 40ml 80ml 160ml

AcOK

KCH₃CO₂, Mw=98.15g/M; (lagern bei NT).

Konz. Stamm. %(w/w) 50ml 100ml 150ml 200ml KC2H3O2 5M 98.15g/M 40.29 24.54g 49.08g 73.61g 98.15g H2O   mQ 59.71 36.36g 72.72g 109.07g 145.43g

5M: p=1.2179.

Konz. Stamm. %(w/w) 10ml 50ml 100ml 150ml 200ml KC2H3O2 1M 98.15g/M 9.38 0.982g 4.908g 9.815g 14.723g 19.63g H2O   mQ 90.62 9.484g 47.42g 94.84g 142.3g 189.7g

1M: p=1.0465.

(AcO)₂Mg

Mg(C₂H₃O₂)₂x 4H₂O; Mw=214.46g/m; (lagern bei NT).

Konz. Stamm. 50ml 100ml 200ml 300ml Mg(C2H3O2)2x4H2O 1M 214.46g/M 10.72g 21.45g 42.89g 64.34g H2O   mQ

1M: 20.44%(w/w) Mg(C₂H₃O₂)₂x4H₂O, 79.56% H₂O; p=1.049.

AcONa

Na(CH₃COO) x 3H₂O; Mw=136.08g/M; (lagern bei NT).

Konz. Stamm. 50ml 100ml 150ml 200ml 300ml AcONax3H2O 3M 136.08g/M 20.41g 40.82g 61.24g 81.65g 122.5g H2O   mQ

Na(CH₃COO); Mw=82.03g/M

Konz. Stamm. 50ml 100ml 150ml 200ml 300ml AcONa 3M 82.03g/M 12.30g 24.61g 36.91g 49.22g 73.83g H2O   mQ

pH5.2 - mit Eisessig einstellen.
pH7.0 - mit verdünnter Essigsäure einstellen.


Kann mit DEPC behandelt werden;

Sterilisation mittels Autoklavieren.





Für 500 ml 3 M NaAc:

Eisessig: pH 5ml 5.90 6ml 5.80 10ml 5.58 20ml 5.25 25ml 5.17 30ml 5.07

Ansetzen von 0.1 M Natrium- und Kaliumacetat Puffer.

pH Lösung A (ml) Lösung B (ml) 3.6 46.3 3.7 3.8 44.0 6.0 4.0 41.0 9.0 4.2 36.8 13.2 4.4 30.5 19.5 4.6 25.5 24.5 4.8 20.0 30.0 5.0 14.8 35.2 5.2 10.5 39.5 5.4 8.8 41.2 5.6 4.8 45.2 Lösung A: 11.5 ml Eisessig/Liter (0.2 M). Lösung B: 0.2 M Natrium-/Kalium Acetat.

CO₃^-

NaHCO₃

Mw=84.01g/M; (lagern bei NT).

Konz. Stamm. 50ml 200ml 300ml NaHCO3 1M 84.01g/M 4.20g 16.80g 25.20g H2O   mQ 48.95ml 195.840ml 293.70ml

1M: p=1.063.

Cl^-

CaCl₂

CaCl₂ Mw=110.98g/M;
CaCl₂x2H₂O Mw=147.02g/M;
CaCl₂x 6H₂O Mw=219.08g/M; (lagern bei NT).

Konz. Stamm. %(w/w) 10ml 50ml 200ml 300ml CaCl2 1M 110.98g/M 10.22 1.110g 5.549g 22.20g 33.29g H2O   mQ 89.78 9.745g 48.73g 194.9g 292.4g

Konz. Stamm. %(w/w) 10ml 50ml 200ml 300ml CaCl2x2H2O 1M 147.02g/M 13.54 1.4702g 7.351g 29.40g 44.11g H2O   mQ 86.46 9.385g 46.92g 187.7g 281.5g

Konz. Stamm. %(w/w) 10ml 50ml 200ml 300ml CaCl2x 6H2O 1M 219.08g/M 20.18 2.191g 10.95g 43.82g 65.72g H2O   mQ 79.82 8.664g 43.32g 173.3g 259.9g

1M: p=1.0855.

KCl

Mw=74.56g/M; (lagern bei NT).

Konz. Stamm. %(w/w) 10ml 50ml 200ml 300ml KCl 1M 74.56g/M 7.14 0.746g 3.728g 14.91g 22.37g H2O   mQ 92.86 9.697g 48.49g 193.9g 290.9g

1M: p=1.0443.

Konz. Stamm. %(w/w) 10ml 50ml 200ml 300ml KCl 2M 74.56g/M 13.8 1.491g 7.46g 29.82g 44.74g H2O   mQ 86.2 9.31ml 46.54g 186.18g 279.3g

2M: p=1.08.
3M: 19.77% (w/w) KCl, 80.23% H₂O; p=1.131.

LiCl

LiCl x H₂O; Mw=60.41g/M; (lagern bei NT).

Konz. Stamm. %(w/w) 50ml 100ml 150ml 200ml LiCl x H2O 9M 60.41g/M 45.52 27.18g 54.37g 81.55g 108.74g H2O   mQ 54.48 32.53g 65.06g 97.59g 130.12g

9M: p=1.1943,
8M: 41.22% (w/w) LiCl x H₂O, 58.78% H₂O; p=1.1724.

MgCl₂

MgCl₂x6H₂O; Mw=203.30g/M; (lagern bei NT).

Konz. Stamm. %(w/w) 10ml 50ml 150ml 200ml MgCl2x6H2O 1M 203.30g/M 18.95 2.033g 10.17g 30.50g 40.66g H2O   mQ 81.05 8.70ml 43.49ml 130.5ml 173.9ml

1M: p=1.073.

MnCl₂

Mw=125.844g/M; (lagern bei NT).

Konz. Stamm. %(w/w) 50ml 100ml 200ml 300ml MnCl2x 4H2O 1M 197.9g/M 18.00 9.895g 19.79g 39.58g 59.37g H2O   mQ 82.00 45.08g 90.15g 180.30g 270.5g

1M: p=1.0994.

NaCl

Mw=58.44g/M; (lagern bei NT).

Konz. Stamm. %(w/w) 10ml 50ml 150ml 200ml NaCl 5M 58.44g/M 24.64 2.922g 14.61g 43.83 58.44g H2O   mQ 75.36 8.937g 44.69g 134.06g 178.74g

5M: p=1.1859.

ZnCl₂

Mw=136.28g/M; (bei 4^oC in gut verschlossenem Gefäß lagern).

Konz. Stamm. %(w/w) 10ml 50ml 100ml 150ml 200ml ZnCl2 1M 136.28g/M 12.27 1.36g 6.81g 13.6g 20.4g 27.3g H2O   mQ 87.73 9.75g 48.7g 97.5g 146g 195g

1M: p=1.111.

Konz. Stamm. 50ml 100ml ZnCl2 3M 136.28g/M 20.44g 40.88g H2O   mQ

SO₄^2-

MgSO₄

MgSO₄x7H₂O; Mw=246.48g/M; (lagern bei NT).

Konz. Stamm. %(w/w) 50ml 100ml 150ml MgSO4x7H2O 1M 246.48g/M 22.17 12.32g 24.65g 36.97g H2O   mQ 77.13 43.28g 86.55g 129.83g

1M: p=1.112.

(NH₄)₂SO₄

Mw=132.1g/M

Konz. Stamm. 50ml (NH4)2SO4 1M 132.1g/M 6.61g H2O   mQ

1M: 12.33% (w/w) (NH₄)₂SO₄, 87.67% H₂O; p=1.071.

ZnSO₄

Mw=287.5g/M

Konz. Stamm. 50ml ZnSO4x7H2O 1M 287.5g/M 14.375g H2O   mQ

1M: 24.90% (w/w) ZnSO₄x7H₂O, 75.10% H₂O; p=1.155.

PO₄^2-

NaP_i

1M (pH 7.2): 6.68% (w/w) Na₂HPO₄, 0.52% (w/w) H₃PO₄, 92.80% H₂O.

Ansetzen von 0.1 M Natrium- und Kaliumphosphat Puffer.

pH SolutionA (ml) Solution B (ml)   pH Solution A (ml) Solution B (ml) 5.7 93.5 6.5   6.9 45.0 55.0 5.8 92.0 8.0   7.0 39.0 61.0 5.9 90.0 10.0   7.1 33.0 67.0 6.0 87.7 12.3   7.2 28.0 72.0 6.1 85.0 15.0   7.3 23.0 77.0 6.2 81.5 18.5   7.4 19.0 81.0 6.3 77.5 22.5   7.5 16.0 84.0 6.4 73.5 26.5   7.6 13.0 87.0 6.5 68.5 31.5   7.7 10.5 90.5 6.6 62.5 37.5   7.8 8.5 91.5 6.7 56.5 43.5   7.9 7.0 93.0 6.8 51.0 49.0   8.0 5.3 94.7 Solution A: 0.2M K/Na H2PO4, Solution B: 0.2M K2/Na2HPO4.

Citrate.

Na citrate

Na₃C₆H₅O₇x2H₂O (lagern bei 4^oC):

Konz. Stamm. 50ml Na3C6H5O7x2H2O 1M 294.1g/M 14.705g H2O   mQ 43.8ml

p=1.17

Isocitrate.

Na isocitrate

Mw=258.1g/M; (lagern bei 4^oC).

Konz. Stamm. % (w/w) 500 µl C6H5O7Na3 1M 258.1g/M 22.35 0.129g H2O   mQ 77.65 448 µl

1M: p=1.155.

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