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Rambler's Top100
AcO-
AcONH4 10M, 7.5M, 2M
AcOK 5M, 1M
(AcO)2Mg 1M
AcONa 3M
pH - Ac K/Na lsungen
CO3-
Cl-
CaCl2 1M
KCl 1M, 2M, 3M
LiCl 9M, 8M
MgCl2 1M
MnCl2 1M
NaCl 5M
ZnCl2 1M, 3M
SO42-
PO42-
citrate
isocitrate
Einige Dichten sind sehr genau angegeben da sie einem chem. Nachschlagewerk entnommen sind.

 
 
 
 
 
 
 
    ber das nochmalige Berechnen von Rezepten fr das gewnschte Volumen:


    AcO-

    AcONH4

    Mw=77.08g/M; (lagern bei NT).
    Konz.Stamm.%(w/w)10ml50ml100ml150ml
    CH3COONH410M77.08g/M71.847.708g38.54g77.08g115.62g
    H2O mQ28.163.022g15.11g30.22g45.33g
    10M: p=1.073.
    Konz.Stamm.50ml100ml200ml
    AcONH47.5M10M37.5ml
    40.24g
    75ml
    80.48g
    150ml
    160.95g

    H2O mQ12.5ml25ml50ml
    Konz.Stamm.50ml100ml200ml
    AcONH42.0M10M10ml
    10.73g
    20ml
    21.46g
    40ml
    42.92g

    H2O mQ40ml80ml160ml

    AcOK

    KCH3CO2, Mw=98.15g/M; (lagern bei NT).
    Konz.Stamm.%(w/w)50ml100ml150ml200ml
    KC2H3O25M98.15g/M40.2924.54g49.08g73.61g98.15g
    H2O mQ59.7136.36g72.72g109.07g145.43g
    5M: p=1.2179.
    Konz.Stamm.%(w/w)10ml50ml100ml150ml200ml
    KC2H3O21M98.15g/M9.380.982g4.908g9.815g14.723g19.63g
    H2O mQ90.629.484g47.42g94.84g142.3g189.7g
    1M: p=1.0465.

    (AcO)2Mg

    Mg(C2H3O2)2x 4H2O; Mw=214.46g/m; (lagern bei NT).
                        Konz.Stamm.50ml100ml200ml300ml
    Mg(C2H3O2)2x4H2O1M214.46g/M10.72g21.45g42.89g64.34g
    H2O mQ     
    Untested information 1M: 20.44%(w/w) Mg(C2H3O2)2x4H2O, 79.56% H2O; p=1.049.

    AcONa

    Na(CH3COO) x 3H2O; Mw=136.08g/M; (lagern bei NT).
    Konz.Stamm.50ml100ml150ml200ml300ml
    AcONax3H2O3M136.08g/M20.41g40.82g61.24g81.65g122.5g
    H2O mQ      
    Na(CH3COO); Mw=82.03g/M
    Konz.Stamm.50ml100ml150ml200ml300ml
    AcONa3M82.03g/M12.30g24.61g36.91g49.22g73.83g
    H2O mQ      
    pH5.2 - mit Eisessig einstellen.
    pH7.0 - mit verdnnter Essigsure einstellen.
  • Kann mit DEPC behandelt werden;
  • Sterilisation mittels Autoklavieren.


  • Fr 500 ml 3 M NaAc:
  • Eisessig:pH
    5ml5.90
    6ml5.80
    10ml5.58
    20ml5.25
    25ml5.17
    30ml5.07

    Ansetzen von 0.1 M Natrium- und Kaliumacetat Puffer.

    pHLsung A (ml)Lsung B (ml)
    3.646.33.7
    3.844.06.0
    4.041.09.0
    4.236.813.2
    4.430.519.5
    4.625.524.5
    4.820.030.0
    5.014.835.2
    5.210.539.5
    5.48.841.2
    5.64.845.2
    Lsung A: 11.5 ml Eisessig/Liter (0.2 M).
    Lsung B: 0.2 M Natrium-/Kalium Acetat.

    CO3-

    NaHCO3

    Mw=84.01g/M; (lagern bei NT).
    Konz.Stamm.50ml200ml300ml
    NaHCO31M84.01g/M4.20g16.80g25.20g
    H2O mQ48.95ml195.840ml293.70ml
    1M: p=1.063.

    Cl-

    CaCl2

    CaCl2 Mw=110.98g/M;
    CaCl2x2H2O Mw=147.02g/M;
    CaCl2x 6H2O Mw=219.08g/M; (lagern bei NT).
    Konz.Stamm.%(w/w)10ml50ml200ml300ml
    CaCl21M110.98g/M10.221.110g5.549g22.20g33.29g
    H2O mQ89.789.745g48.73g194.9g292.4g
    Konz.Stamm.%(w/w)10ml50ml200ml300ml
    CaCl2x2H2O1M147.02g/M13.541.4702g7.351g29.40g44.11g
    H2O mQ86.469.385g46.92g187.7g281.5g
    Konz.Stamm.%(w/w)10ml50ml200ml300ml
    CaCl2x 6H2O1M219.08g/M20.182.191g10.95g43.82g65.72g
    H2O mQ79.828.664g43.32g173.3g259.9g
    1M: p=1.0855.

    KCl

    Mw=74.56g/M; (lagern bei NT).
    Konz.Stamm.%(w/w)10ml50ml200ml300ml
    KCl1M74.56g/M7.140.746g3.728g14.91g22.37g
    H2O mQ92.869.697g48.49g193.9g290.9g
    1M: p=1.0443.
    Konz.Stamm.%(w/w)10ml50ml200ml300ml
    KCl2M74.56g/M13.81.491g7.46g29.82g44.74g
    H2O mQ86.29.31ml46.54g186.18g279.3g
    2M: p=1.08.
    Untested information 3M: 19.77% (w/w) KCl, 80.23% H2O; p=1.131.

    LiCl

    LiCl x H2O; Mw=60.41g/M; (lagern bei NT).
    Konz.Stamm.%(w/w)50ml100ml150ml200ml
    LiCl x H2O9M60.41g/M45.5227.18g54.37g81.55g108.74g
    H2O mQ54.4832.53g65.06g97.59g130.12g
    9M: p=1.1943,
    8M: 41.22% (w/w) LiCl x H2O, 58.78% H2O; p=1.1724.

    MgCl2

    MgCl2x6H2O; Mw=203.30g/M; (lagern bei NT).
    Konz.Stamm.%(w/w)10ml50ml150ml200ml
    MgCl2x6H2O1M203.30g/M18.952.033g10.17g30.50g40.66g
    H2O mQ81.058.70ml43.49ml130.5ml173.9ml
    1M: p=1.073.

    MnCl2

    Mw=125.844g/M; (lagern bei NT).
    Konz.Stamm.%(w/w)50ml100ml200ml300ml
    MnCl2x 4H2O1M197.9g/M18.009.895g19.79g39.58g59.37g
    H2O mQ82.0045.08g90.15g180.30g270.5g
    1M: p=1.0994.

    NaCl

    Mw=58.44g/M; (lagern bei NT).
    Konz.Stamm.%(w/w)10ml50ml150ml200ml
    NaCl5M58.44g/M24.642.922g14.61g43.8358.44g
    H2O mQ75.368.937g44.69g134.06g178.74g
    5M: p=1.1859.

    ZnCl2

    Mw=136.28g/M; (bei 4oC in gut verschlossenem Gef lagern).
    Konz.Stamm.%(w/w)10ml50ml100ml150ml200ml
    ZnCl21M136.28g/M12.271.36g6.81g13.6g20.4g27.3g
    H2O mQ87.739.75g48.7g97.5g146g195g
    1M: p=1.111.
    Konz.Stamm.50ml100ml
    ZnCl23M136.28g/M20.44g40.88g
    H2O mQ   

    SO42-

    MgSO4

    MgSO4x7H2O; Mw=246.48g/M; (lagern bei NT).
    Konz.Stamm.%(w/w)50ml100ml150ml
    MgSO4x7H2O1M246.48g/M22.1712.32g24.65g36.97g
    H2O mQ77.1343.28g86.55g129.83g
    1M: p=1.112.

    (NH4)2SO4

    Mw=132.1g/M
    Konz.Stamm.50ml
    (NH4)2SO41M132.1g/M6.61g
    H2O mQ  
    Untested information 1M: 12.33% (w/w) (NH4)2SO4, 87.67% H2O; p=1.071.

    ZnSO4

    Mw=287.5g/M
    Konz.Stamm.50ml
    ZnSO4x7H2O1M287.5g/M14.375g
    H2O mQ  
    Untested information 1M: 24.90% (w/w) ZnSO4x7H2O, 75.10% H2O; p=1.155.

    PO42-

    NaPi

    Untested information 1M (pH 7.2): 6.68% (w/w) Na2HPO4, 0.52% (w/w) H3PO4, 92.80% H2O.

    Untested information Ansetzen von 0.1 M Natrium- und Kaliumphosphat Puffer.

    pHSolutionA (ml)Solution B (ml) pHSolution A (ml)Solution B (ml)
    5.793.56.5 6.945.055.0
    5.892.08.0 7.039.061.0
    5.990.010.0 7.133.067.0
    6.087.712.3 7.228.072.0
    6.185.015.0 7.323.077.0
    6.281.518.5 7.419.081.0
    6.377.522.5 7.516.084.0
    6.473.526.5 7.613.087.0
    6.568.531.5 7.710.590.5
    6.662.537.5 7.88.591.5
    6.756.543.5 7.97.093.0
    6.851.049.0 8.05.394.7
    Solution A: 0.2M K/Na H2PO4,
    Solution B: 0.2M K2/Na2HPO4.

    Citrate.

    Na citrate

    Na3C6H5O7x2H2O (lagern bei 4oC):
    Konz.Stamm.50ml
    Na3C6H5O7x2H2O1M294.1g/M14.705g
    H2O mQ43.8ml
    p=1.17

    Isocitrate.

    Na isocitrate

    Mw=258.1g/M; (lagern bei 4oC).
    Konz.Stamm.% (w/w)500 µl
    C6H5O7Na31M258.1g/M22.350.129g
    H2O mQ77.65448 µl
    1M: p=1.155.

"Praktische Molekularbiologie" http://www.molbiol.ru/ger
e-mail: editor@molbiol.ru




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