Applications of second-generation sequencing
Материал из Zbio
[править] De novo sequencing
Determining of the primary DNA structure without any preliminary information. Sequencing of new species. De novo assembly is possible for short (~106bp) genomes. Mate-Paired sequencing is very helpfull.
Determining of the primary DNA structure when reference sequence is available. Reference sequence is a similar DNA sequence. In genome biology: genomic sequence of the same (or closely-related) species. Resequencing may be reformulated as analysis of differences between given sample and a reference.
[править] ChIP sequencing
Chromatin immunoprecipitation followed by sequencing. Search for binding sites of DNA-binding enzymes.
[править] Expression profiling
Sequencing of transcriptome. Two tasks: expression profiling and analysis of structural variants: splice-variants, transcript rearangements, etc. May be performed without preliminary DNA analysis, so some resequencing questions also addressed.
[править] microRNA profiling
Sequencing of small-molecular weight transciptome.