Applications of second-generation sequencing

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[править] De novo sequencing

Determining of the primary DNA structure without any preliminary information. Sequencing of new species. De novo assembly is possible for short (~106bp) genomes. Mate-Paired sequencing is very helpfull.


[править] Resequencing

Determining of the primary DNA structure when reference sequence is available. Reference sequence is a similar DNA sequence. In genome biology: genomic sequence of the same (or closely-related) species. Resequencing may be reformulated as analysis of differences between given sample and a reference.


[править] ChIP sequencing

Chromatin immunoprecipitation followed by sequencing. Search for binding sites of DNA-binding enzymes.


[править] Expression profiling

Sequencing of transcriptome. Two tasks: expression profiling and analysis of structural variants: splice-variants, transcript rearangements, etc. May be performed without preliminary DNA analysis, so some resequencing questions also addressed.


[править] microRNA profiling

Sequencing of small-molecular weight transciptome.



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