CBot maintenance

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DECON wash

• should be performed every month
• 5% DECON removes traces of reagents from internal cBot components and inhibits microbial growth
• 5% DECON maybe substituted for 100mM NaOH
• DECON is a highly alkaline wash solution, wear gloves



remove all components before proceeding

  1. water wash
    • from the start screen, touch menu and select manual commands
    • open the commands tab
    • fill the wash reservoir with approximately 12 ml mQ water and touch ?????
    • after the wash is finished, dry the wash reservoir with paper, (don`t rub the outlet ports)

  2. DECON wash
    • fill the wash reservoir with 10 ml of 5% DECON or 100 mM NaOH
    • touch ????? on the screen
    • after the wash is finished, blot out any excess 5% DECON remaining in the wash reservoir with a paper towel (don`t rub the outlet ports)

    NB! do not allow DECON to remain in the wash reservoir for long periods of time as the high pH may corrode the metal pins over repeated, extended contact; dried DECON may clog the wash reservoir holes NB! after removing DECON immediately proceed two times with the water wash
  3. water wash 1
    • fill the wash reservoir with approximately 12 ml deionised water and touch wash
    • after the wash is finished, dry the wash reservoir with paper, (don`t rub the outlet ports)

  4. water wash 2
    • fill the wash reservoir with approximately 12 ml deionised water and touch wash
    • after the wash is finished, dry the wash reservoir with paper, (don`t rub the outlet ports)

  5. sign in the wash sheet at cBot (name + date)
Источник — «http://molbiol.ru/wiki/CBot_maintenance»


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