Preparing reagents for multiplexing (Index Sequencing ) run

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Kit: Multiplexing reagents are provided in the following cluster generation kits:

Single-Read Cluster Generation Kit v2-HS (Catalog # GD-401-2001)
Paired-End Cluster Generation Kit v2-HS (Catalog # PE-401-2001)
    • multiplexing reagents will be used for preparation of the Index Read directly following Read 1.
    • reagents will be loaded onto the PE-rack


Reagents provided in the Multiplexing Sequencing Kit or SBS Kit:
- HP3 (NaOH)
- HT2 (wash buffer)
- HP8 (Index Sequencing primer mix)
- PW1 (provided in the TruSeq SBS Kit)

[править] thaw Reagents:

1. remove HP3, HT2, and HP8 from -20°C storage.
2. thaw reagents in a beaker with RT water

[править] prepare HP3#18:

1. vortex the HP3 tube and pulse spin down.
2. transfer the indicated volume of PW1 into a 15 ml falcon tube and add the indicated volume of HP3.

HiSeq: 3,325µl PW1 and add 175µl of HP3

3. vortex the tube of HP3 dilution, pulse spin down and label the tube with reagent #18.
4. keep the tube at RT until loading on HiSeq

[править] prepare HT2#19:

1. vortex the HT2 and label as reagent #19
2. keep the tube at RT until loading on HiSeq

[править] prepare HP8#17:

1. vortex the HP8 tube, spin down and label as reagent #17
2. keep the tube at RT until loading on HiSeq



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