SOLiD Kits handling

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[править] General

  • mark delivery (or -70°C removal) date on all tubes/bottles
  • label all caps with reagent nick-names
  • use old tubes first
  • mark by cross the last used tube (only one tube should be marked)
  • snap freeze buffers and oligos (NOT ENZYMES!) in liquid nitrogen before storage at -20°C




[править] Library Titration Reagent Kit

      #4392220, 134€, unbalanced

Reagentnickstorageall (portions)
Focal Map P1 LabelFM1-20°C50µl (?)
Focal Map P2 LabelFM235µl (?)
Tag 1 Seq Primer A1A100µl (?)
  • mark delivery date on a box
  • store box at -70°C until the first use
  • label caps: nick and date


[править] Sequencing Probes Kit V2

      #4400412, 4725€, 35bases: 8 slides; PE 2x25bases: 6 slides

Reagentnickstorageall (portions)
Probe Mix AmixA-20°C8x 900µl (8.5x)
  • mark delivery date on a box
  • store box at -70°C until the first use
  • label caps: nick and date




[править] Mate-Paired Library Sequencing Kit V2

      #4400466, 11450€, PE 2x25bases: 6 slide


[править] Mate-Paired Sequencing Buffer Kit

      #4392155, 530€

Reagentnickstorageall (portions)aliquots
20x SSPE BufferSSPENT2ml (x)400µl
Sodium Acetate SolutionAcONa12ml (x)4ml
  • mark delivery date on tubes
  • label caps by nick
  • aliquot before use;


[править] Mate-Paired Sequencing Enzyme Kit

      #4392153, 8900€

Reagentnickstorageall (portions)
10X Phosphatase Buffer10x PB-20°C12ml (x)
Antarctic PhosphatasePhos3x 700µl (x)
T4 DNA LigaseLig8x 1ml (x)
5X T4 DNA Ligase Buffer5x LB4x 13ml (x)
  • mark delivery date on box
  • store box at -70°C until the first use
  • label caps: nick and date
  • mix enzymes carefully and spin-down them for 4 sec
  • minimize freezing-thawing of "T4 DNA Ligase Buffer" as much as possible, aliquot the rest after melting


[править] Mate-Paired Sequencing Oligo Kit V2

      #4400410, 2552€

Reagentnickstorageall (portions)
P1 BlockP1B-20°C300µl (x)
Tag 1 Seq Primer A1A100µl (x)
Tag 1 Seq Primer B1B90µl (x)
Tag 1 Seq Primer C1C2x 45µl (x)
P2 BlockP2B3x 220µl (x)
IA Block 1IA1310µl (x)
IA Block 2IA2310µl (x)
Tag 2 Seq Primer A12A1180µl (x)
Tag 2 Seq Primer A22A2180µl (x)
Tag 2 Seq Primer B12B1180µl (x)
Tag 2 Seq Primer B22B2180µl (x)
Tag 2 Seq Primer C12C190µl (x)
Tag 2 Seq Primer C22C290µl (x)
Focal Map P2 LabelFM22x 35µl (x)
Tag 1 Seq Primer D1D140µl (x)
Tag 1 Seq Primer E1E140µl (x)
Tag 2 Seq Primer D12D190µl (x)
Tag 2 Seq Primer D22D290µl (x)
Tag 2 Seq Primer E12E190µl (x)
Tag 2 Seq Primer E22E290µl (x)
Bridge Probe Tag 1 CB1C450µl (x)
Bridge Probe Tag 1 DB1D450µl (x)
Bridge Probe Tag 1 EB1E450µl (x)
Bridge Probe Tag 2 CB2C300µl (x)
Bridge Probe Tag 2 DB2D300µl (x)
Bridge Probe Tag 2 EB2E300µl (x)
Probe Mix BmixB4x 1550µl (x)
  • mark delivery date on box
  • store box at -70°C until the first use
  • label caps: nick and date




[править] Fragment Library Sequencing Enzyme Kit V2

      #4400467, 8820€, 35bases: 8 slides

[править] Fragment Sequencing Buffer Kit

      #4392165, 530€

Reagentnickstorageall (portions)aliquots
20X SSPE BufferSSPENT2ml (53x)400µl
Sodium Acetate SolutionAcONa12ml (8.5x)4ml
  • mark delivery date on box
  • store box at -70°C until the first use
  • label caps: nick and date
  • aliquot before use


[править] Fragment Sequencing Enzyme Kit

      #4392163, 7600€

Reagentnickstorageall (portions)
10X Phosphatase Buffer10x PB-20°C12ml (7.8x)
Antarctic PhosphatasePhos3x 700µl (8x)
T4 DNA LigaseLig9x 1ml (8.9x)
5X T4 DNA Ligase Buffer5x LB4x 13ml (8.3x)
  • mark delivery date on box
  • store box at -70°C until the first use
  • label caps: nick and date
  • mix enzymes carefully and spin-down them for 4 sec.
  • minimize freezing-thawing of "T4 DNA Ligase Buffer" as much as possible, aliquot the rest after melting


[править] Fragment Sequencing Oligo Kit V2

      #4400411, 1502€

Reagentnickstorageall (portions)
Tag 1 Seq Primer A1A-20°C2x 100µl (13x)
Tag 1 Seq Primer B1B2x 90µl (12x)
Tag 1 Seq Primer C1C3x 45µl (9x)
Tag 1 Seq Primer D1D140µl (9.3x)
Tag 1 Seq Primer E1E140µl (9.3x)
P2 BlockP2B2x 220µl (8.8x)
Focal Map P2 LabelFM23x 35µl (10.5x)
Probe Mix BmixB4x 1550µl (8.5x)
Bridge Probe Tag 1 CB1C450µl (9.9x)
Bridge Probe Tag 1 DB1D450µl (9.3x)
Bridge Probe Tag 1 EB1E450µl (9.3x)
  • mark delivery date on box
  • store box at -70°C until the first use
  • label caps: nick and date




[править] ePCR Kit V2

      #4400834, 2363€, pre-PCR area


[править] ePCR Kit Box 1 of 3

      #4392171

Reagentnickstorageall (portions)1 platealiquots
Emulsion OiloilNT100ml (10,6x)9.45ml50ml portions
Emulsion Stabilizer 1ES120ml (44x)450µl 
Emulsion Stabilizer 2ES22ml (20x)100µl 
MgCl2MgCl24°C2x1ml (14.2x)140µl 
  • mark delivery date
  • label caps
  • aliquot oil before use


[править] ePCR Kit Box 2 of 3

      #4392173, 670€

Reagentnickstorage1 plateall (portions)aliquots
Bead Block SolutionBBS-20°C2x 2ml (20x)160µl650µl
10X PCR Buffer10x PCR2x 5ml (17.8x)560µl 
dNTP MixdNTP2x 4x 1ml (10x)784µl1ml
AmpliTaq Gold DNA pol.A-Taq2x 3ml (10x)600µl0.65g / 600µl (!)
ePCR primer 1ePCR12x 20µl (16x)2.3µl 
ePCR primer 2ePCR22x 170µl (10.1x)34µl 
  • this kit come at 4°C, it should be processed before freezing.
  • Aliquot dNTP's, BBS and A-Taq
  • label caps: nick and date


[править] ePCR Kit Box 3 of 3

      #4392175, 2470€

Reagentnickstorageall (portions)aliquots
P1 DNA BeadsP14°C10x200µl (12.5x)160µl (!)
  • resuspend and aliquot
  • label caps: nick and date




[править] Bead Enrichment Kit

      #4387894, 1654€; post-PCR area

[править] Bead Enrichment Kit Box 1 of 3

      #4392177, 275€

Reagentnickstorageall (portions)1 plate
GlycerolGlycNT15ml (16.6x)0.9ml
Denaturing BufferD.buf20ml (8x)1.98ml
DenaturantDenatur2ml (8x)220µl
  • mark delivery date
  • label caps by nick


[править] Bead Enrichment Kit Box 2 of 3

      #4392179, 365€

Reagentnickstorageall (portions)1 plate
Enrichment OligoEnrich.-20°C50µl (14.3x)3.5µl
  • mark delivery date on box
  • store box at -70°C until the first use
  • mark delivery date on the tube
  • label cap by nick


[править] Bead Enrichment Kit Box 3 of 3

      #4392181, 1580€

Reagentnickstorageall (portions)1 platealiquots
Enrichment BeadsEB4°C3x5ml (38x)650µl650µl and 1.3ml
  • resuspend and aliquot
  • label caps: nick and date




[править] Bead Deposition Kit

      #4387895, 866€; post-PCR area


[править] Bead Deposition Kit 1 of 3

      #4392167, 410€

Reagentnickstorageall (portions)1 plate
10X Term. Transf. Buf.10x TTB-20°C1.5ml (27.3x)44µl
10X Cobalt ChlorideCoCl21.5ml (27.3x)44µl
Terminal TransferaseTT25µl (12.5x)2µl
Bead LinkerB.link50µl (100x)0.5µl
  • mark delivery date on box
  • store box at -70°C until the first use
  • label caps: nick and date


[править] Bead Deposition Kit 2 of 3

      #4392169, 530€

Reagentnickstorageall (portions)
Deposition BufferDepositNT50ml (?x)
  • mark delivery date
  • label caps by nick


[править] Bead Deposition Kit 3 of 3

      overlay buffer 100ml, #4393497, 135€

Reagentnickstorageall (portions)
Overlay BufferOverlay4°C100ml
  • we do not use it




[править] Other kits



[править] Slide Kit

      #4391888, 1355€; post-PCR area

Reagentnickstorageall (portions)
Sequencing Slides, #4389744, 1355€ NT24 slides / box
Slide Storage Buffer, #4389772, 189€Slide buf.3 x 400ml
  • mark delivery date
  • put in desiccator
  • long-term storage: store unpacked at 4°C for
  • working aliquot: at NT in black plastic box with holes


[править] Instrument Buffer Kit

      #4387919, 672€; post-PCR area

Reagentnickstorageall (portions)
Glycerol, #4392215, 48€Glyc.NT2x 900ml (x)
10X Instrument Buffer, #4389784, 71€10x Instr.900ml (x)
Reset Buffer, #4389780, 40€Reset3x 150ml (x)
Cleave Solution 1, #4389776, 40€Cl.14°C6x 72ml (x)
Cleave Solution 2, #4389778, 32€Cl.26x 72ml (x)
Storage Buffer, #4389782, 40€Stor.4x 700ml
  • mark delivery date
  • put alu-foil on caps
  • put fill-marks on the body (Ewset, Cleave and Storage)


[править] Buffer Kit

      #4387918, 236€

Reagentnickstorageall (portions)1 platealiquots
1X Bead Wash Buffer, #4389758, 40€BWBNT40ml (5.7x)2.5ml1.5ml aliquots for pre-PCR
50ml for post-PCR
1X Bind and Wash Buffer, #4389760, 40€Bi-buf25ml (6.25x)2.1ml1.5ml aliquots for pre-PCR
20ml for post-PCR
1X TEX Buffer, #4389766, 95€TEX110ml (13.8x)8ml50ml for pre- and post-PCR
1X Low TE Buffer, #4389764, 32€LTE25ml (100x)250µl1ml aliquots for pre- and post-PCR
1X Low Salt Binding Buffer, #4389762, 32€LS4ml (10x)400µlpost-PCR only
2-Butanol, #4389770, 71€Butanol700ml (41x)12mlpost-PCR only
Nuclease free watermQ 400µl1ml for pre-PCR
20ml for post-PCR
1X TMAX Buffer, #4389768, 40€TMAX20ml (?x)  
  • mark delivery date
  • label caps
  • prepare aliquots before use




[править] Fragment Library kit



[править] Fragment Library Oligo Kit

      #4401151, 275€ The same as "SOLiD Library Oligos Kit 1"


[править] Mate-Paired Library Oligo Kit

      #4400468, 378€


[править] Library Oligos Kit 1

      #4400413, 275€

Reagentnickstorageall (portions)aliquots
P1 Adaptor, 50µM, #4392202Ad1-20°C1ml (x)200µl
P2 Adaptor, 50µM, #4392204Ad21ml (x)200µl
Library PCR Primer 1, 50µM, #4392210PCR11.5ml (x)200µl
Library PCR Primer 2, 50µM, #4392212PCR21.5ml (x)200µl
  • mark delivery date on a box
  • store box at -70°C until the first use
  • label caps: nick and date
  • prepare aliquots before use



[править] Library Oligos Kit 2

      #4400414, 134€

Reagentnickstorageall (portions)aliquots
EcoP 15I CAP Adaptor, 50µM, #4392206Eco CAP-20°C300µl (x)30µl
Internal Adaptor, 2µM, #4392208INT80µl (x)10µl
LMP CAP adaptors, 50µM, #4390505LMP CAP300µl (x)30µl
  • mark delivery date on a box
  • store box at -70°C until the first use
  • label caps: nick and date
  • prepare aliquots before use


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